Recombinant Arabidopsis thaliana ATP-dependent DNA helicase Q-like 2 (RECQL2), partial

Code CSB-YP880823DOA
MSDS
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Source Yeast
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Code CSB-EP880823DOA
MSDS
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Source E.coli
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Code CSB-EP880823DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP880823DOA
MSDS
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Source Baculovirus
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Code CSB-MP880823DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MED34
Uniprot No.
Alternative Names
RECQL2; MED34; MED34_1; RECQ2; RQL2; At1g31360; T19E23.16; ATP-dependent DNA helicase Q-like 2; EC 3.6.4.12; RecQ-like protein 2; AtRQ2; AtRecQ2; AtRecQl2
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
3'-5' DNA helicase that may play a role in the repair of DNA. Its DNA unwinding activity in vitro is dependent on magnesium, and ATP or dATP. Can use GTP/dGTP, CTP/dCTP or UTP/dUTP as nucleotide cofactors. Catalyzes Holliday junction branch migration and replication fork regression. Disrupts D-loop structures.
Gene References into Functions
  1. RecQ helicases AtRECQ2 and AtRECQ3 both show highly repetitive DNA unwinding. Although AtRECQ2 preferentially orients itself towards the junction to unwind it, AtRECQ3 moves in the opposite direction and processively rewinds, that is, helps closing, unzipped hairpins over hundreds of bases. Thus RecQ helicases can exhibit antagonistic activities on the molecular level. [RECQ2] PMID: 23771268
  2. AtRECQ2 is a (d)NTP-dependent 3'-->5' DNA helicase. AtRECQ2 is able to disrupt recombinogenic structures by disrupting various D-loop structures PMID: 18419780
Subcellular Location
Nucleus.
Protein Families
Helicase family, RecQ subfamily
Tissue Specificity
Expressed in shoots and flowers. Expressed in young leaves, inflorescences, roots, shoot apical meristem, young siliques, and mature green siliques.
Database Links

KEGG: ath:AT1G31360

STRING: 3702.AT1G31360.1

UniGene: At.40359

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