SAM2 Antibody

Code CSB-PA323926XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SAM2 Polyclonal antibody
Uniprot No.
Target Names
SAM2
Alternative Names
SAM2 antibody; ETH2 antibody; YDR502C antibody; D9719.8S-adenosylmethionine synthase 2 antibody; AdoMet synthase 2 antibody; EC 2.5.1.6 antibody; Methionine adenosyltransferase 2 antibody; MAT 2 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SAM2 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.
Gene References into Functions
  1. Data showed that co-expression of MET6 and SAM2 improved intracellular methionine availability and redirected the methionine to S-adenosyl-L-methionine biosynthesis. PMID: 26925618
  2. modulation of SAM2 can have different outcomes, from clear effects to no significant phenotypic responses, upon lactic acid stress in different genetic backgrounds PMID: 25359316
  3. SAM2 was successfully produced at high level by the recombinant methylotrophic yeast Pichia pastoris, and the secreted His6-tagged SAM synthetase was purified in a single chromatography step with a yield of approximately 82% for the total activity. PMID: 18078345
Protein Families
AdoMet synthase family
Database Links

KEGG: sce:YDR502C

STRING: 4932.YDR502C

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