Recombinant Saccharomyces cerevisiae S-adenosylmethionine synthase 2 (SAM2)

Code CSB-YP323926SVG
MSDS
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Source Yeast
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Code CSB-EP323926SVG
MSDS
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Source E.coli
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Code CSB-EP323926SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP323926SVG
MSDS
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Source Baculovirus
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Code CSB-MP323926SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SAM2
Uniprot No.
Alternative Names
SAM2; ETH2; YDR502C; D9719.8S-adenosylmethionine synthase 2; AdoMet synthase 2; EC 2.5.1.6; Methionine adenosyltransferase 2; MAT 2
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
2-384
Target Protein Sequence
SKSKTFLFT SESVGEGHPD KICDQVSDAI LDACLEQDPF SKVACETAAK TGMIMVFGEI TTKARLDYQQ IVRDTIKKIG YDDSAKGFDY KTCNVLVAIE QQSPDIAQGL HYEKSLEDLG AGDQGIMFGY ATDETPEGLP LTILLAHKLN MAMADARRDG SLPWLRPDTK TQVTVEYEDD NGRWVPKRID TVVISAQHAD EISTADLRTQ LQKDIVEKVI PKDMLDENTK YFIQPSGRFV IGGPQGDAGL TGRKIIVDAY GGASSVGGGA FSGKDYSKVD RSAAYAARWV AKSLVAAGLC KRVQVQFSYA IGIAEPLSLH VDTYGTATKS DDEIIEIIKK NFDLRPGVLV KELDLARPIY LPTASYGHFT NQEYSWEKPK KLEF
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.
Gene References into Functions
  1. Data showed that co-expression of MET6 and SAM2 improved intracellular methionine availability and redirected the methionine to S-adenosyl-L-methionine biosynthesis. PMID: 26925618
  2. modulation of SAM2 can have different outcomes, from clear effects to no significant phenotypic responses, upon lactic acid stress in different genetic backgrounds PMID: 25359316
  3. SAM2 was successfully produced at high level by the recombinant methylotrophic yeast Pichia pastoris, and the secreted His6-tagged SAM synthetase was purified in a single chromatography step with a yield of approximately 82% for the total activity. PMID: 18078345
Protein Families
AdoMet synthase family
Database Links

KEGG: sce:YDR502C

STRING: 4932.YDR502C

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