MID1 Antibody

Code CSB-PA331352XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MID1 Polyclonal antibody
Uniprot No.
Target Names
MID1
Alternative Names
MID1 antibody; YNL291C antibody; N0530 antibody; Stretch-activated cation channel MID1 antibody; Mating pheromone-induced death protein 2 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MID1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Calcium-permeable, cation-selective stretch-activated channel (SAC). Required for calcium influx and for vitality of MATa cells in a late, pheromone-induced event of the mating process requiring calcium induced signaling. Functions together with CCH1 to ensure that adequate levels of Ca(2+) are supplied to PMR1 to sustain secretion and growth. Required for growth in low-calcium environments. Together with CCH1, essential for tolerance to iron stress, which leads to an increased oxidative poise, and to cold stress.
Gene References into Functions
  1. Cch1 and Mid1 mediate the ethanol-induced Ca2+ flux. PMID: 21259000
Subcellular Location
Cell membrane. Endoplasmic reticulum. Membrane; Single-pass type I membrane protein. Note=Trafficking to the plasma membrane is dependent on the N-glycosylation and the transporter protein SEC12.
Database Links

KEGG: sce:YNL291C

STRING: 4932.YNL291C

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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