Product Details

Full Product Name

Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) mid1 Polyclonal antibody

Uniprot No.

P78953

Target Names

mid1

Alternative Names

mid1 antibody; dmf1 antibody; SPCC4B3.15 antibody; Division mal foutue 1 protein antibody

Raised in

Rabbit

Species Reactivity

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Immunogen

Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) mid1 protein

Immunogen Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Scaffold protein that anchors the contractile ring (CR) at the cell equator during cytokinesis. At the onset of mitosis, membrane-bound oligomers of mid1 assemble recruitment platforms for cytokinetic ring components at the medial cortex and stabilize the ring position during its compaction. Recruits dephosphorylated myo2, but also rng2, clp1 and cdc15 to nodes and to place cytokinetic nodes around the cell equator the medial cortex to promote the ring assembly in cooperation with F-actin. Necessary to stabilize the mitotic spindle perpendicular to the axis of cell division. Recruits also the cdr2 kinase to the CR.; In the nucleus, binds to the promoter regions of M-G1 transcribed genes to negatively regulate their expression.

Gene References into Functions

Unlike in wild-type cells, actomyosin rings in cells rewired to divide medially in the absence of Mid1p assemble late in anaphase. The rewiring process affects the ability of the actomyosin ring to track the nucleus upon perturbation of nuclear position. PMID: 25176634
Pom1 inhibits Cdr2 interaction with Mid1, reducing its clustering ability. PMID: 24982431
An overview of the studies that have deciphered how Mid1 localization and scaffolding activities are controlled over the cell cycle to ensure the symmetrical division of fission yeast cells, is provided. PMID: 22888038
Orderly assembly of the contractile ring in wild-type cells depends on Mid1p. PMID: 22918943
Residues 1-578 of Mid1p assemble node components myosin-II, Rng2p, and Cdc15p, and the insoluble domain facilitates the normal, efficient condensation of nodes into rings. PMID: 22918954
Blt1 acts as a scaffolding protein for precursors of the cytokinetic ring, and Blt1 and Mid1 provide overlapping membrane anchors for proper division plane positioning. PMID: 23149940
The N-terminus of Mid1 physically interacts with cytokinesis node proteins. PMID: 22427686
Clp1p and Mid1p form links between cell cycle progression and gene expression at cytokinesis in fission yeast. PMID: 21512312
Mid1p recruits Rng2p to cortical nodes at the cell-division site. PMID: 21376595
Live microscopy of cells expressing Cdc12p fluorescent fusion proteins shows for the first time that Cdc12p localizes to a broad band of 30-50 dynamic nodes, where actin filaments are nucleated in random directions. PMID: 19864459
Evidence indicates that Clp1/Flp1 must interact with the Mid1 scaffold to ensure the fidelity of Schizosaccharomyces pombe cytokinesis. PMID: 18378776
Data show that Mid1p/anillin and the septation initiation network orchestrate contractile ring assembly for cytokinesis. PMID: 19056897
A mechanism that is independent of cortical nodes is sufficient for the assembly of normal actomyosin rings. PMID: 19075108

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Subcellular Location

Nucleus. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton.

Database Links

KEGG: spo:SPCC4B3.15

STRING: 4896.SPCC4B3.15.1

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mid1 Antibody

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