Product Details

Purity

>85% (SDS-PAGE)

Target Names

mid1

Uniprot No.

P78953

Alternative Names

mid1; dmf1; SPCC4B3.15; Division mal foutue 1 protein

Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Related Products

Target Background

Function

Scaffold protein that anchors the contractile ring (CR) at the cell equator during cytokinesis. At the onset of mitosis, membrane-bound oligomers of mid1 assemble recruitment platforms for cytokinetic ring components at the medial cortex and stabilize the ring position during its compaction. Recruits dephosphorylated myo2, but also rng2, clp1 and cdc15 to nodes and to place cytokinetic nodes around the cell equator the medial cortex to promote the ring assembly in cooperation with F-actin. Necessary to stabilize the mitotic spindle perpendicular to the axis of cell division. Recruits also the cdr2 kinase to the CR.; In the nucleus, binds to the promoter regions of M-G1 transcribed genes to negatively regulate their expression.

Gene References into Functions

Unlike in wild-type cells, actomyosin rings in cells rewired to divide medially in the absence of Mid1p assemble late in anaphase. The rewiring process affects the ability of the actomyosin ring to track the nucleus upon perturbation of nuclear position. PMID: 25176634
Pom1 inhibits Cdr2 interaction with Mid1, reducing its clustering ability. PMID: 24982431
An overview of the studies that have deciphered how Mid1 localization and scaffolding activities are controlled over the cell cycle to ensure the symmetrical division of fission yeast cells, is provided. PMID: 22888038
Orderly assembly of the contractile ring in wild-type cells depends on Mid1p. PMID: 22918943
Residues 1-578 of Mid1p assemble node components myosin-II, Rng2p, and Cdc15p, and the insoluble domain facilitates the normal, efficient condensation of nodes into rings. PMID: 22918954
Blt1 acts as a scaffolding protein for precursors of the cytokinetic ring, and Blt1 and Mid1 provide overlapping membrane anchors for proper division plane positioning. PMID: 23149940
The N-terminus of Mid1 physically interacts with cytokinesis node proteins. PMID: 22427686
Clp1p and Mid1p form links between cell cycle progression and gene expression at cytokinesis in fission yeast. PMID: 21512312
Mid1p recruits Rng2p to cortical nodes at the cell-division site. PMID: 21376595
Live microscopy of cells expressing Cdc12p fluorescent fusion proteins shows for the first time that Cdc12p localizes to a broad band of 30-50 dynamic nodes, where actin filaments are nucleated in random directions. PMID: 19864459
Evidence indicates that Clp1/Flp1 must interact with the Mid1 scaffold to ensure the fidelity of Schizosaccharomyces pombe cytokinesis. PMID: 18378776
Data show that Mid1p/anillin and the septation initiation network orchestrate contractile ring assembly for cytokinesis. PMID: 19056897
A mechanism that is independent of cortical nodes is sufficient for the assembly of normal actomyosin rings. PMID: 19075108

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Subcellular Location

Nucleus. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton.

Database Links

KEGG: spo:SPCC4B3.15

STRING: 4896.SPCC4B3.15.1

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Recombinant Schizosaccharomyces pombe Division mal foutue 1 protein (mid1), partial

Product Details

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