MLO7 Antibody

Code CSB-PA519513XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MLO7 Polyclonal antibody
Uniprot No.
Target Names
MLO7
Alternative Names
MLO7; NTA; At2g17430; F5J6.19; MLO-like protein 7; AtMlo7; Protein NORTIA
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana MLO7 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
May be involved in modulation of pathogen defense and leaf cell death. Activity seems to be regulated by Ca(2+)-dependent calmodulin binding and seems not to require heterotrimeric G proteins. Controls pollen tube reception in the female gametophyte synergids.
Gene References into Functions
  1. critical role in pollen tube reception dependent on localization within synergids into a Golgi-associated compartment before pollen tube arrival, indicating that pollen tube-triggered regulation of the synergid secretory system is important for synergid function during pollination PMID: 28724621
  2. study shows pollen tube (PT) reception and powdery mildew (PM) infection share molecular components; NTA, discovered in context of PM resistance, and FERONIA control PT reception; fer mutants also display PM resistance revealing a new function for FER PMID: 21071669
Subcellular Location
Cell membrane; Multi-pass membrane protein. Endomembrane system. Note=In synergids of unfertilized mature female gametophytes, present in a punctate pattern of endomembranes throughout the cytoplasm. Become polarly localized to the basal half of the synergids, to the filiform apparatus, upon pollen tube arrival at the micropyle. This relocalization upon fertilization requires FER.
Protein Families
MLO family
Tissue Specificity
Restricted to pollen, synergids, pistils and immature anthers. Also detected in seedlings, leaves, stems and inflorescens.
Database Links

KEGG: ath:AT2G17430

STRING: 3702.AT2G17430.1

UniGene: At.28692

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