Recombinant Arabidopsis thaliana MLO-like protein 7 (MLO7)

Code CSB-CF519513DOA
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
MLO7
Uniprot No.
Alternative Names
MLO7; NTA; At2g17430; F5J6.19; MLO-like protein 7; AtMlo7; Protein NORTIA
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-542
Target Protein Sequence
MITRSRCRRSLLWFLVFHGGATATGAPSGGKELSQTPTWAVAVVCTFLILISHLLEKGLQ RLANWLWKKHKNSLLEALEKIKAELMILGFISLLLTFGEPYILKICVPRKAALSMLPCLS EDTVLFQKLAPSSLSRHLLAAGDTSINCKQGSEPLITLKGLHQLHILLFFLAIFHIVYSL ITMMLSRLKIRGWKKWEQETLSNDYEFSIDHSRLRLTHETSFVREHTSFWTTTPFFFYVG CFFRQFFVSVERTDYLTLRHGFISAHLAPGRKFNFQRYIKRSLEDDFKLVVGISPVLWAS FVIFLLFNVNGWRTLFWASIPPLLIILAVGTKLQAIMATMALEIVETHAVVQGMPLVQGS DRYFWFDCPQLLLHLIHFALFQNAFQITHFFWIWYSFGLKSCFHKDFNLVVSKLFLCLGA LILCSYITLPLYALVTQMGSHMKKAVFDEQMAKALKKWHKDIKLKKGKARKLPSKTLGVS ESFSLSSSSSATTLHRSKTTGHSSNIIYYKQEDEEDEMSDLEAGAEDAIDRIQQQEMQFH NS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May be involved in modulation of pathogen defense and leaf cell death. Activity seems to be regulated by Ca(2+)-dependent calmodulin binding and seems not to require heterotrimeric G proteins. Controls pollen tube reception in the female gametophyte synergids.
Gene References into Functions
  1. critical role in pollen tube reception dependent on localization within synergids into a Golgi-associated compartment before pollen tube arrival, indicating that pollen tube-triggered regulation of the synergid secretory system is important for synergid function during pollination PMID: 28724621
  2. study shows pollen tube (PT) reception and powdery mildew (PM) infection share molecular components; NTA, discovered in context of PM resistance, and FERONIA control PT reception; fer mutants also display PM resistance revealing a new function for FER PMID: 21071669
Subcellular Location
Cell membrane; Multi-pass membrane protein. Endomembrane system. Note=In synergids of unfertilized mature female gametophytes, present in a punctate pattern of endomembranes throughout the cytoplasm. Become polarly localized to the basal half of the synergids, to the filiform apparatus, upon pollen tube arrival at the micropyle. This relocalization upon fertilization requires FER.
Protein Families
MLO family
Tissue Specificity
Restricted to pollen, synergids, pistils and immature anthers. Also detected in seedlings, leaves, stems and inflorescens.
Database Links

KEGG: ath:AT2G17430

STRING: 3702.AT2G17430.1

UniGene: At.28692

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