Map1lc3b Antibody

Code CSB-PA717270XA01RA
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Product Details

Full Product Name
Rabbit anti-Rattus norvegicus (Rat) Map1lc3b Polyclonal antibody
Uniprot No.
Target Names
Map1lc3b
Alternative Names
Map1lc3b antibody; Map1alc3 antibody; Map1lc3 antibody; Microtubule-associated proteins 1A/1B light chain 3B antibody; Autophagy-related protein LC3 B antibody; Autophagy-related ubiquitin-like modifier LC3 B antibody; MAP1 light chain 3-like protein 2 antibody; MAP1A/MAP1B light chain 3 B antibody; MAP1A/MAP1B LC3 B antibody; Microtubule-associated protein 1 light chain 3 beta antibody
Raised in
Rabbit
Species Reactivity
Rattus norvegicus (Rat)
Immunogen
Recombinant Rattus norvegicus (Rat) Map1lc3b protein
Immunogen Species
Rattus norvegicus (Rat)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.
Gene References into Functions
  1. Quantifying autophagy using novel LC3B and p62 TR-FRET assays. PMID: 29554128
  2. a novel role of activated LC3B induced by hyperoxia in AECII transdifferentiation PMID: 27187184
  3. microtubule-associated protein 1 light chain 3 binds to cardiolipin. Preventing this interaction inhibits rotenone-induced mitochondrial delivery to autophagosomes PMID: 24351649
  4. Lipidation of the LC3/GABARAP family of autophagy proteins relies on a membrane-curvature-sensing domain in Atg3. PMID: 24747438
  5. Data show that caldendrin specifically associates with the microtubule cytoskeleton via an interaction with the microtubule componentlight chain 3 (Mpl3). PMID: 15095872

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Subcellular Location
Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor.
Protein Families
ATG8 family
Tissue Specificity
Abundant only in neurons. Detected in testes.
Database Links

KEGG: rno:64862

UniGene: Rn.41412

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