stg Antibody

Code CSB-PA321809XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) stg Polyclonal antibody
Uniprot No.
Target Names
stg
Alternative Names
stg antibody; cdc25 antibody; CG1395 antibody; M-phase inducer phosphatase antibody; EC 3.1.3.48 antibody; Cdc25-like protein antibody; Protein string antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) stg protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
This protein functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It may directly dephosphorylate Cdk1 and activate the Cdk1 activity.
Gene References into Functions
  1. Premature removal of cdc25 transcripts by RNAi did not affect progression to the midblastula transition. PMID: 23290551
  2. The degradation rate of String protein gradually increases during the late syncytial cycles in a manner dependent on the nuclear-to-cytoplasmic ratio and on the DNA replication checkpoints. PMID: 23290553
  3. findings suggest that high Cdc25 and Cdk1 contribute to the speed of the rapid, pre-midblastula transition (MBT) S phases and that down-regulation of these activities plays a broader role in MBT-associated changes than was previously suspected PMID: 22431511
  4. String (Cdc25) regulates stem cell maintenance, proliferation and aging in Drosophila testis. PMID: 22031544
  5. Pointed, an activator, and Tramtrack69, a repressor, directly regulate the transcription of string and thereby cell proliferation PMID: 12447387
  6. Induction of stg rescues the neuroblast proliferation defect in trol mutant animals. PMID: 12929089
  7. String and dappled were identified as being important for the proper formation of adult external sensory (ES) organs. PMID: 18420029
  8. Thereby, we show that abdominal histoblasts, which grow while in G2 arrest during larval stages, enter a proliferative stage in the pupal period that is initiated by ecdysone-dependent string/Cdc25 phosphatase transcription. PMID: 19355788

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Protein Families
MPI phosphatase family
Database Links

KEGG: dme:Dmel_CG1395

STRING: 7227.FBpp0084766

UniGene: Dm.3208

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