Recombinant Drosophila melanogaster M-phase inducer phosphatase (stg)

Code CSB-YP321809DLU
MSDS
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Source Yeast
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Code CSB-EP321809DLU
MSDS
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Source E.coli
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Code CSB-EP321809DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP321809DLU
MSDS
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Source Baculovirus
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Code CSB-MP321809DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
stg
Uniprot No.
Alternative Names
stg; cdc25; CG1395; M-phase inducer phosphatase; EC 3.1.3.48; Cdc25-like protein; Protein string
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-479
Target Protein Sequence
MLWETIVEEN NCSMDCNISN NTSSSSSINK MSGSRRARRS LELMSMDQEE LSFYDDDVVP QDQQRSASPE LMGLLSPEGS PQRFQIVRQP KILPAMGVSS DHTPARSFRI FNSLSSTCSM ESSMDDEYME LFEMESQSQQ TALGFPSGLN SLISGQIKEQ PAAKSPAGLS MRRPSVRRCL SMTESNTNST TTPPPKTPET ARDCFKRPEP PASANCSPIQ SKRHRCAAVE KENCPAPSPL SQVTISHPPP LRKCMSLNDA EIMSALARSE NRNEPELIGD FSKAYALPLM EGRHRDLKSI SSETVARLLK GEFSDKVASY RIIDCRYPYE FEGGHIEGAK NLYTTEQILD EFLTVQQTEL QQQQNAESGH KRNIIIFHCE FSSERGPKMS RFLRNLDRER NTNAYPALHY PEIYLLHNGY KEFFESHVEL CEPHAYRTML DPAYNEAYRH FRAKSKSWNG DGLGGATGRL KKSRSRLML
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
This protein functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It may directly dephosphorylate Cdk1 and activate the Cdk1 activity.
Gene References into Functions
  1. Premature removal of cdc25 transcripts by RNAi did not affect progression to the midblastula transition. PMID: 23290551
  2. The degradation rate of String protein gradually increases during the late syncytial cycles in a manner dependent on the nuclear-to-cytoplasmic ratio and on the DNA replication checkpoints. PMID: 23290553
  3. findings suggest that high Cdc25 and Cdk1 contribute to the speed of the rapid, pre-midblastula transition (MBT) S phases and that down-regulation of these activities plays a broader role in MBT-associated changes than was previously suspected PMID: 22431511
  4. String (Cdc25) regulates stem cell maintenance, proliferation and aging in Drosophila testis. PMID: 22031544
  5. Pointed, an activator, and Tramtrack69, a repressor, directly regulate the transcription of string and thereby cell proliferation PMID: 12447387
  6. Induction of stg rescues the neuroblast proliferation defect in trol mutant animals. PMID: 12929089
  7. String and dappled were identified as being important for the proper formation of adult external sensory (ES) organs. PMID: 18420029
  8. Thereby, we show that abdominal histoblasts, which grow while in G2 arrest during larval stages, enter a proliferative stage in the pupal period that is initiated by ecdysone-dependent string/Cdc25 phosphatase transcription. PMID: 19355788

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Protein Families
MPI phosphatase family
Database Links

KEGG: dme:Dmel_CG1395

STRING: 7227.FBpp0084766

UniGene: Dm.3208

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