Product Details

Full Product Name

Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) pals1a Polyclonal antibody

Uniprot No.

Q8JHF4

Target Names

pals1a

Alternative Names

mpp5a antibody; mpp5 antibody; nok antibody; MAGUK p55 subfamily member 5-A antibody; MAGUK family factor antibody; Nagie oko protein antibody

Raised in

Rabbit

Species Reactivity

Danio rerio (Zebrafish) (Brachydanio rerio)

Immunogen

Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) pals1a protein

Immunogen Species

Danio rerio (Zebrafish) (Brachydanio rerio)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Plays a role in tight junction biogenesis and in the establishment of cell polarity in epithelial cells. Also involved in adherens junction biogenesis. Functions in cellular patterning of the retina.

Gene References into Functions

This study shows that Spatial-temporal expressions of Nagie oko and their interdependence in zebrafish central nervous system during early development. PMID: 24071007
The structural disorganization of the pronephric glomerulus in the mpp5a(m520) mutant is likely to result from circulatory failure, rather than the anomaly of Mpp5a protein in the glomerulus. PMID: 23027442
the ratio of the number of cells undergoing neurogenic cell division to the total number of cells undergoing mitosis was decreased in retinas of nagie oko knockout zebrafish. PMID: 20362667
morphogenesis of the neural tube requires nok function PMID: 15788456
Data show that nagie oko contains a predicted nuclear export and two conserved nuclear localization signals and loss of the predicted nuclear export signal results in nuclear protein accumulation. PMID: 18058913
deletion constructs of Nok in functional rescue experiments to define domains essential for cell polarity, maintenance of epithelial integrity and cardiac morphogenesis.Nok does not interact with Crumbs proteins upon deletion of the PDZ domain PMID: 18628301
that Nok is required for retinal pigment epithelium integrity in a tissue-autonomous manner PMID: 19074041

Hide All

Subcellular Location

Apical cell membrane; Peripheral membrane protein. Cell junction, tight junction. Note=Localizes to the apical region of the cell membrane, in the vicinity of junctional complexes in the neuroepithelium and the photoreceptor layer. Enriched in the areas immediately apical to the adherens junctions-associated actin bundles.

Protein Families

MAGUK family

Tissue Specificity

Expressed in the retina and in the neural tube.

Database Links

KEGG: dre:252845

STRING: 7955.ENSDARP00000078936

UniGene: Dr.18838

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA816939XA01DIL
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

pals1a Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background