Recombinant Danio rerio MAGUK p55 subfamily member 5-A (mpp5a), partial

Code CSB-YP816939DIL
MSDS
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Source Yeast
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Code CSB-EP816939DIL
MSDS
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Source E.coli
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Code CSB-EP816939DIL-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP816939DIL
MSDS
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Source Baculovirus
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Code CSB-MP816939DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mpp5a
Uniprot No.
Alternative Names
mpp5a; mpp5; nok; MAGUK p55 subfamily member 5-A; MAGUK family factor; Nagie oko protein
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a role in tight junction biogenesis and in the establishment of cell polarity in epithelial cells. Also involved in adherens junction biogenesis. Functions in cellular patterning of the retina.
Gene References into Functions
  1. This study shows that Spatial-temporal expressions of Nagie oko and their interdependence in zebrafish central nervous system during early development. PMID: 24071007
  2. The structural disorganization of the pronephric glomerulus in the mpp5a(m520) mutant is likely to result from circulatory failure, rather than the anomaly of Mpp5a protein in the glomerulus. PMID: 23027442
  3. the ratio of the number of cells undergoing neurogenic cell division to the total number of cells undergoing mitosis was decreased in retinas of nagie oko knockout zebrafish. PMID: 20362667
  4. morphogenesis of the neural tube requires nok function PMID: 15788456
  5. Data show that nagie oko contains a predicted nuclear export and two conserved nuclear localization signals and loss of the predicted nuclear export signal results in nuclear protein accumulation. PMID: 18058913
  6. deletion constructs of Nok in functional rescue experiments to define domains essential for cell polarity, maintenance of epithelial integrity and cardiac morphogenesis.Nok does not interact with Crumbs proteins upon deletion of the PDZ domain PMID: 18628301
  7. that Nok is required for retinal pigment epithelium integrity in a tissue-autonomous manner PMID: 19074041

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Subcellular Location
Apical cell membrane; Peripheral membrane protein. Cell junction, tight junction. Note=Localizes to the apical region of the cell membrane, in the vicinity of junctional complexes in the neuroepithelium and the photoreceptor layer. Enriched in the areas immediately apical to the adherens junctions-associated actin bundles.
Protein Families
MAGUK family
Tissue Specificity
Expressed in the retina and in the neural tube.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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