MURJ Antibody

Datasheet

Code	CSB-PA365235XA01ENV
Size	US$299
Image	Western Blot Positive WB detected in Recombinant protein All lanes: MURJ antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 16.8 kDa Observed band size: 17 kDa
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) murJ Polyclonal antibody

Uniprot No.

P0AF16

Target Names

murJ

Alternative Names

murJ antibody; mviN antibody; yceN antibody; b1069 antibody; JW1056 antibody; Lipid II flippase MurJ antibody; Peptidoglycan biosynthesis protein MurJ antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) MURJ protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein A/G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The MURJ antibody is produced by immunizing rabbits with the recombinant MURJ protein from Escherichia coli (strain K12). It undergoes purification through protein A/G affinity chromatography. This MURJ antibody is suitable for detecting the MURJ protein of Escherichia coli (strain K12) in ELISA and WB experiments.

The Escherichia coli (strain K12) MURJ protein belongs to the Mur ligase family and is involved in the biosynthesis of peptidoglycan, a major component of the bacterial cell wall. It is believed to play a role in the synthesis of lipid intermediates required for peptidoglycan assembly.

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Target Background

Function

Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane.

Gene References into Functions

The crystal structure of substrate-free MurJ reveals an inward-open conformation. High-throughput mutagenesis and next-generation sequencing assessed mutational tolerance at every amino acid in the protein, providing a detailed functional and structural map for the enzyme and identifying sites for inhibitor development. A rocker-switch model for lipid II transport was supported. PMID: 29891673
From these data, authors hypothesize that some charged residues in the cavity region of MurJ homologs are required for interaction with lipid II and/or energy coupling during transport. PMID: 25225268
MurJ is the lipid II flippase in Escherichia coli. PMID: 25013077

Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Protein Families

MurJ/MviN family

Database Links

KEGG: ecj:JW1056

STRING: 316385.ECDH10B_1140

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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MURJ Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background