Nbr Antibody

Code CSB-PA894699XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Nbr Polyclonal antibody
Uniprot No.
Target Names
Nbr
Alternative Names
Nbr antibody; CG9247 antibody; Exonuclease mut-7 homolog antibody; EC 3.1.-.- antibody; Exonuclease 3'-5' domain-containing protein 3 homolog antibody; Protein nibbler antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Nbr protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Possesses 3'-5' exoribonuclease activity. Required for 3'-end trimming of AGO1-bound miRNAs, in particular multiple-isoform miRNAs, which represents a critical step in miRNA maturation.
Gene References into Functions
  1. Nbr is expressed only during a short time of oogenesis and appears ubiquitously localized within oocytes. Nbr was is not enriched in the nuage where it was shown to be involved in piwi-mediated mechanisms. PMID: 28974923
  2. antagonistic roles of Nbr and Hen1 define a mechanism to modulate piRNA 3' ends. PMID: 26718004
  3. indicate a role of Nibbler on age-associated processes and to modulate length of multiple classes of small RNAs including miRNAs and piRNAs in Drosophila PMID: 25754031
  4. An RNA interference screen identified a putative 3'-5' exoribonuclease CG9247/nibbler essential for the generation of smaller isoforms of the microRNA miR-34. Nibbler interacts with Argonaute1 and processes miR-34 within the RNA-induced silencing complex. PMID: 22055292
  5. The study provides a molecular explanation for the previously reported heterogeneity of miRNA 3' ends and proposes a model in which Nibbler (CG9247)converts miRNAs into isoforms that are compatible with the preferred length of Ago1-bound small RNAs. PMID: 22055293

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Protein Families
Mut-7 family
Database Links

KEGG: dme:Dmel_CG9247

STRING: 7227.FBpp0081027

UniGene: Dm.30864

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