Recombinant Drosophila melanogaster Probable exonuclease mut-7 homolog (CG9247), partial

Code CSB-YP894699DLU
MSDS
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Source Yeast
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Code CSB-EP894699DLU
MSDS
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Source E.coli
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Code CSB-EP894699DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP894699DLU
MSDS
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Source Baculovirus
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Code CSB-MP894699DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nbr
Uniprot No.
Alternative Names
Nbr; CG9247; Exonuclease mut-7 homolog; EC 3.1.-.-; Exonuclease 3'-5' domain-containing protein 3 homolog; Protein nibbler
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Possesses 3'-5' exoribonuclease activity. Required for 3'-end trimming of AGO1-bound miRNAs, in particular multiple-isoform miRNAs, which represents a critical step in miRNA maturation.
Gene References into Functions
  1. Nbr is expressed only during a short time of oogenesis and appears ubiquitously localized within oocytes. Nbr was is not enriched in the nuage where it was shown to be involved in piwi-mediated mechanisms. PMID: 28974923
  2. antagonistic roles of Nbr and Hen1 define a mechanism to modulate piRNA 3' ends. PMID: 26718004
  3. indicate a role of Nibbler on age-associated processes and to modulate length of multiple classes of small RNAs including miRNAs and piRNAs in Drosophila PMID: 25754031
  4. An RNA interference screen identified a putative 3'-5' exoribonuclease CG9247/nibbler essential for the generation of smaller isoforms of the microRNA miR-34. Nibbler interacts with Argonaute1 and processes miR-34 within the RNA-induced silencing complex. PMID: 22055292
  5. The study provides a molecular explanation for the previously reported heterogeneity of miRNA 3' ends and proposes a model in which Nibbler (CG9247)converts miRNAs into isoforms that are compatible with the preferred length of Ago1-bound small RNAs. PMID: 22055293

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Protein Families
Mut-7 family
Database Links

KEGG: dme:Dmel_CG9247

STRING: 7227.FBpp0081027

UniGene: Dm.30864

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