MYB12 Antibody

Code CSB-PA519496XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MYB12 Polyclonal antibody
Uniprot No.
Target Names
MYB12
Alternative Names
MYB12 antibody; PFG1 antibody; At2g47460 antibody; T30B22.24 antibody; Transcription factor MYB12 antibody; Myb-related protein 12 antibody; AtMYB12 antibody; Protein PRODUCTION OF FLAVONOL GLYCOSIDES 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana MYB12 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Flavonol-specific transcription activator involved in the regulation of several genes of flavonoid biosynthesis. Activates the expression of CHS, CHI, F3H and FLS1. Controls flavonol biosynthesis mainly in the root. Confers tolerance to UV-B.
Gene References into Functions
  1. The R2R3-MYB transcription factor MYB12 (AtMYB12) activation domain (AD) was identified in the amino acid region 273 to 325 (of 371), with the region 309-325 having the most relevant effect on activation capacity. PMID: 28284041
  2. Results showed that AtMYB12-overexpressing Arabidopsis plants exhibited increased levels of flavonoids and enhanced salt and drought tolerance suggesting that overexpression of AtMYB12 up-regulates genes involved in flavonoid biosynthesis, abscisic acid biosynthesis, proline biosynthesis and ROS-scavenging system, resulting in significant physiological changes. PMID: 27033553
  3. results demonstrate that modulation of primary carbon metabolism and other pathways by AtMYB12 in tomato may lead to sufficient substrate supply for enhanced content of phenolics in general and flavonols in particular. PMID: 26206248
  4. AtMYB12 was overexpressed in a commercial cultivar of kale and the transgenic plants were tested both in in vitro and in semi-field conditions. A severalfold increase in both total phenolics content and flavonol accumulation was achieved. PMID: 24792422
  5. The quantity and diversity of flavonoids is increased by overexpressing MYB12/PAP1. Flavonoids enhances both biotic and abiotic stress tolerance in crops. PMID: 24274116
  6. The characterization of MYB12 as a flavonol-specific activator of flavonoid biosynthesis in A. thaliana is reported. PMID: 15923334

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in stems and flower buds. Expressed in seedlings, roots, cotyledons and apical meristems.
Database Links

KEGG: ath:AT2G47460

STRING: 3702.AT2G47460.1

UniGene: At.10899

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