Recombinant Arabidopsis thaliana Transcription factor MYB12 (MYB12)

Code CSB-YP519496DOA
MSDS
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Source Yeast
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Code CSB-EP519496DOA
MSDS
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Source E.coli
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Code CSB-EP519496DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP519496DOA
MSDS
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Source Baculovirus
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Code CSB-MP519496DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MYB12
Uniprot No.
Alternative Names
MYB12; PFG1; At2g47460; T30B22.24; Transcription factor MYB12; Myb-related protein 12; AtMYB12; Protein PRODUCTION OF FLAVONOL GLYCOSIDES 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-371
Target Protein Sequence
MGRAPCCEKV GIKRGRWTAE EDQILSNYIQ SNGEGSWRSL PKNAGLKRCG KSCRLRWINY LRSDLKRGNI TPEEEELVVK LHSTLGNRWS LIAGHLPGRT DNEIKNYWNS HLSRKLHNFI RKPSISQDVS AVIMTNASSA PPPPQAKRRL GRTSRSAMKP KIHRTKTRKT KKTSAPPEPN ADVAGADKEA LMVESSGAEA ELGRPCDYYG DDCNKNLMSI NGDNGVLTFD DDIIDLLLDE SDPGHLYTNT TCGGDGELHN IRDSEGARGF SDTWNQGNLD CLLQSCPSVE SFLNYDHQVN DASTDEFIDW DCVWQEGSDN NLWHEKENPD SMVSWLLDGD DEATIGNSNC ENFGEPLDHD DESALVAWLL S
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Flavonol-specific transcription activator involved in the regulation of several genes of flavonoid biosynthesis. Activates the expression of CHS, CHI, F3H and FLS1. Controls flavonol biosynthesis mainly in the root. Confers tolerance to UV-B.
Gene References into Functions
  1. The R2R3-MYB transcription factor MYB12 (AtMYB12) activation domain (AD) was identified in the amino acid region 273 to 325 (of 371), with the region 309-325 having the most relevant effect on activation capacity. PMID: 28284041
  2. Results showed that AtMYB12-overexpressing Arabidopsis plants exhibited increased levels of flavonoids and enhanced salt and drought tolerance suggesting that overexpression of AtMYB12 up-regulates genes involved in flavonoid biosynthesis, abscisic acid biosynthesis, proline biosynthesis and ROS-scavenging system, resulting in significant physiological changes. PMID: 27033553
  3. results demonstrate that modulation of primary carbon metabolism and other pathways by AtMYB12 in tomato may lead to sufficient substrate supply for enhanced content of phenolics in general and flavonols in particular. PMID: 26206248
  4. AtMYB12 was overexpressed in a commercial cultivar of kale and the transgenic plants were tested both in in vitro and in semi-field conditions. A severalfold increase in both total phenolics content and flavonol accumulation was achieved. PMID: 24792422
  5. The quantity and diversity of flavonoids is increased by overexpressing MYB12/PAP1. Flavonoids enhances both biotic and abiotic stress tolerance in crops. PMID: 24274116
  6. The characterization of MYB12 as a flavonol-specific activator of flavonoid biosynthesis in A. thaliana is reported. PMID: 15923334

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in stems and flower buds. Expressed in seedlings, roots, cotyledons and apical meristems.
Database Links

KEGG: ath:AT2G47460

STRING: 3702.AT2G47460.1

UniGene: At.10899

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