NARL Antibody

Rare Species
Code CSB-PA360208XA01ENV
Size US$299
Image
  • Western Blot
    Positive WB detected in Recombinant protein
    All lanes: NARL antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 35.5 kDa
    Observed band size: 34 kDa
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) narL Polyclonal antibody
Uniprot No.
Target Names
narL
Alternative Names
narL antibody; frdR antibody; b1221 antibody; JW1212 antibody; Nitrate/nitrite response regulator protein NarL antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) NARL protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein A/G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The NARL antibody is produced by immunizing rabbits with a recombinant NARL protein derived from Escherichia coli (strain K12). Afterward, it is purified using protein A/G affinity chromatography from the rabbit serum. This specific NARL antibody is capable of detecting the NARL protein of Escherichia coli (strain K12) in ELISA and WB applications.

The Escherichia coli (strain K12) NARL is a gene locus that is associated with nitrate and nitrite reduction pathways in E. coli. The NARL protein is believed to play a role in the regulation or assembly of nitrate and nitrite reductase enzymes, which are involved in the anaerobic respiration of nitrate and nitrite.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
This protein activates the expression of the nitrate reductase (narGHJI) and formate dehydrogenase-N (fdnGHI) operons and represses the transcription of the fumarate reductase (frdABCD) operon in response to a nitrate/nitrite induction signal transmitted by either the NarX or NarQ proteins.
Gene References into Functions
  1. The NarL receiver domain operates as an on-off switch to occlude or release the output domain in a nitrate-responsive manner. PMID: 26307095
  2. NarL and NarP carboxyl-terminal domains activate transcription from Nar class II control regions. PMID: 20634237
  3. Data describe two cocrystal structures of NarL complexed with additional 20mer oligonucleotides representative of other high-affinity tail-to-tail NarL binding sites found in upstream promoter regions. PMID: 16262254
  4. binding of NarL/NarP appears to insulate the nrf promoter from the effects of integration host factor PMID: 16936015
  5. DNA-bound dimers of phospho-NarL can participate in higher-order cooperative interac PMID: 17965164
  6. Analysis of the DNA sequence of the ydhY promoter region (PydhY) revealed the presence of four heptameric sequences similar to NarL/P binding sites PMID: 18227264
  7. DNase I footprinting studies identified Fis-binding sites that overlap the yeaR promoter NarL site at position -43.5, and the ogt promoter NarL site at position -78.5, and suggest that Fis represses both promoters by displacing NarL. PMID: 19245365
  8. The authors show that the global transcription factor FNR is a class II activator at the ynfEFGHI promoter and that NarL represses activation by binding to a single target that overlaps the promoter -10 element. PMID: 19251855

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