Recombinant Escherichia coli Nitrate/nitrite response regulator protein NarL (narL)

Code CSB-YP360208ENV
MSDS
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Source Yeast
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Code CSB-EP360208ENV
MSDS
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Source E.coli
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Code CSB-EP360208ENV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP360208ENV
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP360208ENV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
narL
Uniprot No.
Alternative Names
narL; frdR; b1221; JW1212; Nitrate/nitrite response regulator protein NarL
Species
Escherichia coli (strain K12)
Expression Region
1-216
Target Protein Sequence
MSNQEPATIL LIDDHPMLRT GVKQLISMAP DITVVGEASN GEQGIELAES LDPDLILLDL NMPGMNGLET LDKLREKSLS GRIVVFSVSN HEEDVVTALK RGADGYLLKD MEPEDLLKAL HQAAAGEMVL SEALTPVLAA SLRANRATTE RDVNQLTPRE RDILKLIAQG LPNKMIARRL DITESTVKVH VKHMLKKMKL KSRVEAAVWV HQERIF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
This protein activates the expression of the nitrate reductase (narGHJI) and formate dehydrogenase-N (fdnGHI) operons and represses the transcription of the fumarate reductase (frdABCD) operon in response to a nitrate/nitrite induction signal transmitted by either the NarX or NarQ proteins.
Gene References into Functions
  1. The NarL receiver domain operates as an on-off switch to occlude or release the output domain in a nitrate-responsive manner. PMID: 26307095
  2. NarL and NarP carboxyl-terminal domains activate transcription from Nar class II control regions. PMID: 20634237
  3. Data describe two cocrystal structures of NarL complexed with additional 20mer oligonucleotides representative of other high-affinity tail-to-tail NarL binding sites found in upstream promoter regions. PMID: 16262254
  4. binding of NarL/NarP appears to insulate the nrf promoter from the effects of integration host factor PMID: 16936015
  5. DNA-bound dimers of phospho-NarL can participate in higher-order cooperative interac PMID: 17965164
  6. Analysis of the DNA sequence of the ydhY promoter region (PydhY) revealed the presence of four heptameric sequences similar to NarL/P binding sites PMID: 18227264
  7. DNase I footprinting studies identified Fis-binding sites that overlap the yeaR promoter NarL site at position -43.5, and the ogt promoter NarL site at position -78.5, and suggest that Fis represses both promoters by displacing NarL. PMID: 19245365
  8. The authors show that the global transcription factor FNR is a class II activator at the ynfEFGHI promoter and that NarL represses activation by binding to a single target that overlaps the promoter -10 element. PMID: 19251855

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