NARX Antibody

Datasheet

Code	CSB-PA365278XA01ENV
Size	US$299
Image	Western Blot Positive WB detected in Recombinant protein All lanes: NARX antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40.1 kDa Observed band size: 44 kDa
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) narX Polyclonal antibody

Uniprot No.

P0AFA2

Target Names

narX

Alternative Names

narX; narR; b1222; JW1213; Nitrate/nitrite sensor protein NarX

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) NARX protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein A/G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The NARX antibody is produced by immunizing rabbits with a recombinant NARX protein derived from Escherichia coli (strain K12). It undergoes purification using protein A/G affinity chromatography. This specific NARX antibody is designed for detecting the NARX protein of Escherichia coli (strain K12) in ELISA and WB assays. The Escherichia coli (strain K12) NARX protein is believed to be involved in the regulation of gene expression and cellular processes.

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Target Background

Function

Acts as a sensor for nitrate/nitrite and transduces signal of nitrate availability to the NarL protein and of both nitrate/nitrite to the NarP protein. NarX probably activates NarL and NarP by phosphorylation in the presence of nitrate. NarX also plays a negative role in controlling NarL activity, probably through dephosphorylation in the absence of nitrate.

Gene References into Functions

Results from bacterial adenylate cyclase two-hybrid analysis revealed robust interactions for the NarX-NarL, NarQ-NarL and NarQ-NarP pairs but a much weaker interaction for the NarX-NarP pair. PMID: 25873583
Missense substitutions in NarX affect transmitter phosphatase function without directly changing the active-site region. PMID: 23517441
HAMP domain HD2 and S-helix regions overlap and form a continuous coiled-coil marked by a heptad repeat stutter. Impaired induction phenotypes were conferred by heptad deletions within the S-helix conserved core and that remove the heptad stutter. PMID: 19966007
analysis of FNR regulon and its interactions with nitrate, nitrite, NarXL, and NarQP PMID: 16377617
study showed that only a small proportion of NarX and NarQ remain phosphorylated in the absence of nitrate, resulting in efficient response regulator dephosphorylation by the remaining unphosphorylated sensors PMID: 18375557

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Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Database Links

KEGG: ecj:JW1213

STRING: 316385.ECDH10B_1281

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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NARX Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background