Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ABH1 Polyclonal antibody

Uniprot No.

Q9SIU2

Target Names

ABH1

Alternative Names

ABH1 antibody; CBP80 antibody; ENS antibody; At2g13540 antibody; T10F5.8 antibody; Nuclear cap-binding protein subunit 1 antibody; 80 kDa nuclear cap-binding protein antibody; AtCBP80 antibody; NCBP 80 kDa subunit antibody; Abscisic acid-hypersensitive protein 1 antibody; ABA-hypersensitive protein 1 antibody; Protein ENSALADA antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana ABH1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing and RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs). The CBC complex is involved in miRNA-mediated RNA interference and is required for primary miRNA processing. In the CBC complex, ABH1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure. Involved in flowering regulation, possibly by regulating pre-mRNA splicing of FLC gene. Acts as a negative regulator of abscisic acid signaling in guard cells.

Gene References into Functions

the identification of new genes involved in ABA-dependent abiotic stresses using Arabidopsis suppressor mutants of abh1 hypersensitivity to ABA during seed germination. PMID: 23807502
Data suggest that CBP20 and CBP80 (ABH1) have synergistic roles during the salt stress response. PMID: 24689873
SERRATE, CAP BINDING PROTEIN20 (CBP20), and CBP80 are regulators of lincRNA expression and biogenesis. RT-PCR experiments confirmed these three proteins are also needed for splicing of a small group of intron-containing lincRNAs. PMID: 23136377
This intron suppression of transgene silencing requires efficient intron splicing and is dependent on ABH1. PMID: 21689169
ABH1 (CBP80) encodes a large subunit of CBC (CAP BINDING COMPLEX) and the abh1 mutant is drought-tolerant and hypersensitive to ABA during seed germination. PMID: 23196831
The cbp80(abh1) and cbp20/80 mutants had many more changes in alternative splicing in common than did cbp20 and cbp20/80 suggesting that CBP80 plays a more significant role in alternative splicing than CBP20. PMID: 19864257
Abh1 lesion affects several developmental processes, perhaps because the processing of certain mRNAs. PMID: 15361145
CBP20 and CBP80 function in miRNA biogenesis by binding to pri-miRNA transcripts, and are stabilized by ABA by a post-translational mechanism. PMID: 18829588
AtCBP80 is required for the stability of AtCBP20 in the plant cell. PMID: 19453442

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Subcellular Location

Nucleus. Cytoplasm. Note=Predominantly nuclear.

Protein Families

NCBP1 family

Tissue Specificity

Expressed in all tissues analyzed, including roots, stems, leaves and flowers.

Database Links

KEGG: ath:AT2G13540

STRING: 3702.AT2G13540.1

UniGene: At.25306

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ABH1 Antibody

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