Recombinant Arabidopsis thaliana Nuclear cap-binding protein subunit 1 (ABH1), partial

Code CSB-YP882871DOA
MSDS
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Source Yeast
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Code CSB-EP882871DOA
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Source E.coli
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Code CSB-EP882871DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP882871DOA
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Source Baculovirus
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Code CSB-MP882871DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ABH1
Uniprot No.
Alternative Names
ABH1; CBP80; ENS; At2g13540; T10F5.8; Nuclear cap-binding protein subunit 1; 80 kDa nuclear cap-binding protein; AtCBP80; NCBP 80 kDa subunit; Abscisic acid-hypersensitive protein 1; ABA-hypersensitive protein 1; Protein ENSALADA
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing and RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs). The CBC complex is involved in miRNA-mediated RNA interference and is required for primary miRNA processing. In the CBC complex, ABH1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure. Involved in flowering regulation, possibly by regulating pre-mRNA splicing of FLC gene. Acts as a negative regulator of abscisic acid signaling in guard cells.
Gene References into Functions
  1. the identification of new genes involved in ABA-dependent abiotic stresses using Arabidopsis suppressor mutants of abh1 hypersensitivity to ABA during seed germination. PMID: 23807502
  2. Data suggest that CBP20 and CBP80 (ABH1) have synergistic roles during the salt stress response. PMID: 24689873
  3. SERRATE, CAP BINDING PROTEIN20 (CBP20), and CBP80 are regulators of lincRNA expression and biogenesis. RT-PCR experiments confirmed these three proteins are also needed for splicing of a small group of intron-containing lincRNAs. PMID: 23136377
  4. This intron suppression of transgene silencing requires efficient intron splicing and is dependent on ABH1. PMID: 21689169
  5. ABH1 (CBP80) encodes a large subunit of CBC (CAP BINDING COMPLEX) and the abh1 mutant is drought-tolerant and hypersensitive to ABA during seed germination. PMID: 23196831
  6. The cbp80(abh1) and cbp20/80 mutants had many more changes in alternative splicing in common than did cbp20 and cbp20/80 suggesting that CBP80 plays a more significant role in alternative splicing than CBP20. PMID: 19864257
  7. Abh1 lesion affects several developmental processes, perhaps because the processing of certain mRNAs. PMID: 15361145
  8. CBP20 and CBP80 function in miRNA biogenesis by binding to pri-miRNA transcripts, and are stabilized by ABA by a post-translational mechanism. PMID: 18829588
  9. AtCBP80 is required for the stability of AtCBP20 in the plant cell. PMID: 19453442

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Subcellular Location
Nucleus. Cytoplasm. Note=Predominantly nuclear.
Protein Families
NCBP1 family
Tissue Specificity
Expressed in all tissues analyzed, including roots, stems, leaves and flowers.
Database Links

KEGG: ath:AT2G13540

STRING: 3702.AT2G13540.1

UniGene: At.25306

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