NCE2 Antibody

Datasheet

Code	CSB-PA614189XA01SVG
Size	US$299
Image	Western Blot Positive WB detected in Recombinant protein All lanes: NCE102 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 20.1 kDa Observed band size: 22 kDa
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Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) NCE102 Polyclonal antibody

Uniprot No.

Q12207

Target Names

NCE102

Alternative Names

NCE102; NCE2; RTG2S2; YPR149W; P9659.2; Non-classical export protein 2

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) NCE2 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein A/G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The rabbit receives an injection of the recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) NCE2 protein to stimulate its immune system. Antibodies against the NCE2 protein are produced by the rabbit's immune system over time. Once a satisfactory antibody response is generated, a serum sample is collected to extract the polyclonal antibodies. The NCE2 antibody undergoes protein A/G affinity chromatography purification. The effectiveness of the NCE2 antibody is assessed through ELISA and WB applications. This specific NCE2 antibody exhibits the ability to specifically react with the Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) NCE2 protein.

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Target Background

Function

Involved in membrane organization. Required for the formation of membrane compartments of CAN1 (MCCs), localization of CAN1 at the MCCs and subsequent invagination of the plasma membrane at the MCCs sites. Involved in eisosome organization and might act as a sensor of sphingolipids that regulates plasma membrane function. Involved in a novel pathway of export of proteins that lack a cleavable signal sequence. It may be an accessory subunit to an essential core component of the non-classical export machinery. Non-classical export pathway functions also as an alternative clearance/detoxification pathway to eliminate damaged material, when the basic repair pathway is not sufficient.

Gene References into Functions

Attenuation of the endocytic pathway through deletion of the gene END3 impaired the Nce102-mediated rescue, suggesting that the Nce102 mutants lead to suppression through the yeast endocytic pathway. PMID: 27317660
Overexpression of NCE102 in wild type yeast strain BY4742 neither protected against oxidative stress due to diethylmaleate nor extended yeast lifespan compared to its parental wild type strain. PMID: 17415679
Results suggest that Nce102 might act as a sensor of sphingolipids that regulates plasma membrane function. PMID: 19564405

Subcellular Location

Cell membrane; Multi-pass membrane protein. Note=Associates with the ergosterol-rich membrane compartment of CAN1 (MCC). Accumulates in membrane domains at eisosomes.

Protein Families

NCE102 family

Database Links

KEGG: sce:YPR149W

STRING: 4932.YPR149W

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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NCE2 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background