NAP1 Antibody

Code CSB-PA719335XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NAP1 Polyclonal antibody
Uniprot No.
Target Names
NAP1
Alternative Names
NAP1 antibody; GRL antibody; NAP antibody; NAPP antibody; At2g35110 antibody; T4C15.22 antibody; Protein NAP1 antibody; ARP2/3 regulatory protein subunit NAPP antibody; NAP of plants antibody; Nck-associated protein 1 antibody; AtNAP1 antibody; AtNAP125 antibody; Protein GNARLED antibody; p125Nap1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana NAP1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in regulation of actin and microtubule organization. Part of a WAVE complex that activates the Arp2/3 complex.
Gene References into Functions
  1. NAP1, a component of the SCAR/WAVE complex, locates to vesicles or puncta that appear upon applied pressure. The majority co-localizes with the autophagosome marker ATG8, and anti-NAP1 identifies autophagosomes in immuno-TEM. Fewer autophagosomes are generated in the NAP1 knockout mutant during starvation stress. PMID: 27451899
  2. NAP1 localizes to multiple organelles in developing pavement cells and trichomes. The endoplasmic reticulum-associated NAP1 is inactive because it displays little colocalization with the actin network. PMID: 23613272
  3. encodes a putative regulator of the WAVE-HSPC300 complex, NAP125, combined with the phenotypic similarity between arp2/3 and grl mutants, provides evidence that the ARP2/3 complex is indeed regulated by the above mentioned pathway in Arabidopsis [gnarled] PMID: 15368121
  4. The multiple growth and developmental phenotypes of Atnap and Atpir mutants reveals these proteins are also required for a wider variety of cellular functions in addition to regulating trichome cell growth. PMID: 15516496
  5. AtNAP1;1 is a component of a regulatory mechanism that connects cell proliferation to cell growth and expansion during Arabidopsis leaf development. PMID: 17041028

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Protein Families
HEM-1/HEM-2 family
Tissue Specificity
Expressed in roots, root hairs, hypocotyls, cotyledons, stems, leaves, trichomes, and flowers.
Database Links

KEGG: ath:AT2G35110

STRING: 3702.AT2G35110.1

UniGene: At.22860

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