Recombinant Arabidopsis thaliana Protein NAP1 (NAP1), partial

Code CSB-YP719335DOA
MSDS
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Source Yeast
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Code CSB-EP719335DOA
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Source E.coli
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Code CSB-EP719335DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP719335DOA
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Source Baculovirus
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Code CSB-MP719335DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NAP1
Uniprot No.
Alternative Names
NAP1; GRL; NAP; NAPP; At2g35110; T4C15.22; Protein NAP1; ARP2/3 regulatory protein subunit NAPP; NAP of plants; Nck-associated protein 1; AtNAP1; AtNAP125; Protein GNARLED; p125Nap1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in regulation of actin and microtubule organization. Part of a WAVE complex that activates the Arp2/3 complex.
Gene References into Functions
  1. NAP1, a component of the SCAR/WAVE complex, locates to vesicles or puncta that appear upon applied pressure. The majority co-localizes with the autophagosome marker ATG8, and anti-NAP1 identifies autophagosomes in immuno-TEM. Fewer autophagosomes are generated in the NAP1 knockout mutant during starvation stress. PMID: 27451899
  2. NAP1 localizes to multiple organelles in developing pavement cells and trichomes. The endoplasmic reticulum-associated NAP1 is inactive because it displays little colocalization with the actin network. PMID: 23613272
  3. encodes a putative regulator of the WAVE-HSPC300 complex, NAP125, combined with the phenotypic similarity between arp2/3 and grl mutants, provides evidence that the ARP2/3 complex is indeed regulated by the above mentioned pathway in Arabidopsis [gnarled] PMID: 15368121
  4. The multiple growth and developmental phenotypes of Atnap and Atpir mutants reveals these proteins are also required for a wider variety of cellular functions in addition to regulating trichome cell growth. PMID: 15516496
  5. AtNAP1;1 is a component of a regulatory mechanism that connects cell proliferation to cell growth and expansion during Arabidopsis leaf development. PMID: 17041028

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Protein Families
HEM-1/HEM-2 family
Tissue Specificity
Expressed in roots, root hairs, hypocotyls, cotyledons, stems, leaves, trichomes, and flowers.
Database Links

KEGG: ath:AT2G35110

STRING: 3702.AT2G35110.1

UniGene: At.22860

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