NCL Antibody

Datasheet

Code	CSB-PA797796XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NCL Polyclonal antibody

Uniprot No.

Q8L636

Target Names

NCL

Alternative Names

NCL antibody; At1g53210 antibody; F12M16.12 antibody; Sodium/calcium exchanger NCL antibody; Na(+)/Ca(2+)-exchange protein NCL antibody; Protein NCX-like antibody; AtNCL antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana NCL protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

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Target Background

Function

Possesses sodium/calcium exchanger (NCX) activity when expressed in a heterologous mammalian CHO-K1 cell system. Does not possess cation/proton exchanger (CAX) or sodium/proton (NHX) activity when expressed in a heterologous yeast cell system. Has the ability to bind calcium in vitro. Participates in the maintenance of calcium homeostasis. May play a role in auxin response, diurnal rhythm and flowering time. Involved in salt stress response.

Gene References into Functions

AtNCL is a vacuolar Ca(2+) -regulated Na(+) /Ca(2+) exchanger that regulates auxin responses and flowering time. PMID: 26296956
AtNCL (At1g53210)participates in the maintenance of Ca(2+) homeostasis. PMID: 23148213

Subcellular Location

Cell membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein.

Protein Families

Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family

Tissue Specificity

Expressed in roots, leaves, stems, petals, stamens, ovules and siliques.

Database Links

KEGG: ath:AT1G53210

STRING: 3702.AT1G53210.1

UniGene: At.23754

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