Ndc80 Antibody

Code CSB-PA875172XA01MO
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Product Details

Full Product Name
Rabbit anti-Mus musculus (Mouse) Ndc80 Polyclonal antibody
Uniprot No.
Target Names
Ndc80
Alternative Names
Ndc80 antibody; Hec1 antibody; Kntc2 antibody; Kinetochore protein NDC80 homolog antibody; Kinetochore protein Hec1 antibody; Kinetochore-associated protein 2 antibody
Raised in
Rabbit
Species Reactivity
Mus musculus (Mouse)
Immunogen
Recombinant Mus musculus (Mouse) Ndc80 protein
Immunogen Species
Mus musculus (Mouse)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein A/G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules.
Gene References into Functions
  1. These data strongly indicated that KNTC2 is a promising target for the treatment of HCC and that phosphorylated HH3 at serine 10 is one of the target engagement markers for KNTC2. PMID: 28843857
  2. Further investigation of the phosphoregulation of meiotic Kif4 revealed that Aurora Kinase and Cdk activity is critical for Kif4 kinetochore localization and interaction with Ndc80 and CENP-C. Finally, Kif4 protein but not gene expression was found to be upregulated with age, suggesting a role for this protein in the decline of oocyte quality with age PMID: 28125646
  3. Hec1-dependent cyclin B2 stabilization during early metaphase is important for early-stage spindle assembly. PMID: 23541922
  4. Disruption of Ndc80 function by either siRNA injection or antibody injection resulted in severe chromosome misalignment, spindle disruption, and precocious polar body extrusion. PMID: 21600073
  5. overexpression of Hec1 results in mitotic checkpoint hyperactivation that leads to aneuploidy sufficient to generate tumors PMID: 18940925

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Subcellular Location
Nucleus. Chromosome, centromere, kinetochore.
Protein Families
NDC80/HEC1 family
Tissue Specificity
Expressed in spleen, testis and thymus.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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