Recombinant Mouse Kinetochore protein NDC80 homolog (Ndc80)

Code CSB-YP875172MO
MSDS
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Source Yeast
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Code CSB-EP875172MO
MSDS
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Source E.coli
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Code CSB-EP875172MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP875172MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP875172MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ndc80
Uniprot No.
Alternative Names
Ndc80; Hec1; Kntc2; Kinetochore protein NDC80 homolog; Kinetochore protein Hec1; Kinetochore-associated protein 2
Species
Mus musculus (Mouse)
Expression Region
1-642
Target Protein Sequence
MKRSSVSTCG AGRLSMQELR TLDLNKPGLY TPQTKERSTF GKLSTHKPTS ERKVSIFGKR TSGHGSRNSQ LGIFSSSEKI KDPRPLNDKA FIQQCIRQLY EFLTENGYVY SVSMKSLQAP STKEFLKIFA FLYGFLCPSY ELPGTKCEEE VPRIFKALGY PFTLSKSSMY TVGAPHTWPH IVAALVWLID CIKIDTAMKE SSPLFDDGQL WGEETEDGIK HNKLFLEYTK KCYEKFMTGA DSFEEEDAEL QAKLKDLYKV DASKLESLEA ENKALNEQIA RLEEEREREP NRLMSLKKLK ASLQADVQNY KAYMSNLESH LAVLKQKSNS LDEEIGRVEQ ECETVKQENT RLQSIVDNQK YSVADIERIN HEKNELQQTI NKLTKDLEAE QQQMWNEELK YARGKEAIEA QLAEYHKLAR KLKLIPKGAE NSKGYDFEIK FNPEAGANCL VKYRTQVYAP LKELLNESEE EINKALNKKR HLEDTLEQLN TMKTESKNTV RMLKEEIQKL DDLHQQAVKE AEEKDKKSAS ELESLEKHKH LLESGVNDGL SEAMDELDAV QREYQLTVKT TTEERRKVEN NLQRLLEMVA THVGSLEKHL EEENAKADRE YEEFMSEDLL ENIREMAEKY KRNAAQLKAP DK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules.
Gene References into Functions
  1. These data strongly indicated that KNTC2 is a promising target for the treatment of HCC and that phosphorylated HH3 at serine 10 is one of the target engagement markers for KNTC2. PMID: 28843857
  2. Further investigation of the phosphoregulation of meiotic Kif4 revealed that Aurora Kinase and Cdk activity is critical for Kif4 kinetochore localization and interaction with Ndc80 and CENP-C. Finally, Kif4 protein but not gene expression was found to be upregulated with age, suggesting a role for this protein in the decline of oocyte quality with age PMID: 28125646
  3. Hec1-dependent cyclin B2 stabilization during early metaphase is important for early-stage spindle assembly. PMID: 23541922
  4. Disruption of Ndc80 function by either siRNA injection or antibody injection resulted in severe chromosome misalignment, spindle disruption, and precocious polar body extrusion. PMID: 21600073
  5. overexpression of Hec1 results in mitotic checkpoint hyperactivation that leads to aneuploidy sufficient to generate tumors PMID: 18940925

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Subcellular Location
Nucleus. Chromosome, centromere, kinetochore.
Protein Families
NDC80/HEC1 family
Tissue Specificity
Expressed in spleen, testis and thymus.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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