FRO1 Antibody

Code CSB-PA887780XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) FRO1 Polyclonal antibody
Uniprot No.
Target Names
FRO1
Alternative Names
FRO1 antibody; At5g67590 antibody; K9I9.16 antibody; NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 antibody; mitochondrial antibody; Protein FROSTBITE1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana FRO1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Gene References into Functions
  1. Mitochondrial perturbations related to a defect in complex I in the Arabidopsis thaliana frostbite1 (fro1) mutant, carrying a point mutation in the 8-kD Fe-S subunit of NDUFS4 protein, alter aspects of fundamental carbon metabolism, which is manifested as stunted growth. The responses of fro1 plants to NH treatment were consistent with the inhibition of a form of programmed cell death. PMID: 30060552
  2. The growth of fro1 plants increased under ammonium nutrition. Ammonium nutrition increased the reduction level of pyridine nucleotides in the leaves of wild-type plants, but not in the leaves of fro1 mutant plants. PMID: 25040883
  3. Removal of the single protein subunit NDUFS4 prevents assembly of complex I of the respiratory chain and removes its function from mitochondria without pleiotropic effects on other respiratory components. [NDUFS4] PMID: 19675153
Subcellular Location
Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Protein Families
Complex I NDUFS4 subunit family
Database Links

KEGG: ath:AT5G67590

STRING: 3702.AT5G67590.1

UniGene: At.28805

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