Recombinant Arabidopsis thaliana NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (FRO1)

Code CSB-YP887780DOA
MSDS
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Source Yeast
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Code CSB-EP887780DOA
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Source E.coli
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Code CSB-EP887780DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887780DOA
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Source Baculovirus
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Code CSB-MP887780DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FRO1
Uniprot No.
Alternative Names
FRO1; At5g67590; K9I9.16; NADH dehydrogenase [ubiquinone] iron-sulfur protein 4; mitochondrial; Protein FROSTBITE1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
25-154
Target Protein Sequence
ATDAVV ESDYKRGEIG KVSGIPEEHL SRKVIIYSPA RTATQSGSGK LGKWKINFVS TLKWENPLMG WTSTGDPYAN VGDSALAFDS EEAAKSFAER HGWDYKVKKP NTPLLKVKSY SDNFKWKGNP QPEN
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Gene References into Functions
  1. Mitochondrial perturbations related to a defect in complex I in the Arabidopsis thaliana frostbite1 (fro1) mutant, carrying a point mutation in the 8-kD Fe-S subunit of NDUFS4 protein, alter aspects of fundamental carbon metabolism, which is manifested as stunted growth. The responses of fro1 plants to NH treatment were consistent with the inhibition of a form of programmed cell death. PMID: 30060552
  2. The growth of fro1 plants increased under ammonium nutrition. Ammonium nutrition increased the reduction level of pyridine nucleotides in the leaves of wild-type plants, but not in the leaves of fro1 mutant plants. PMID: 25040883
  3. Removal of the single protein subunit NDUFS4 prevents assembly of complex I of the respiratory chain and removes its function from mitochondria without pleiotropic effects on other respiratory components. [NDUFS4] PMID: 19675153
Subcellular Location
Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Protein Families
Complex I NDUFS4 subunit family
Database Links

KEGG: ath:AT5G67590

STRING: 3702.AT5G67590.1

UniGene: At.28805

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