Nek8 Antibody

Code CSB-PA849695XA01MO
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Product Details

Full Product Name
Rabbit anti-Mus musculus (Mouse) Nek8 Polyclonal antibody
Uniprot No.
Target Names
Nek8
Alternative Names
Nek8 antibody; Jck antibody; Serine/threonine-protein kinase Nek8 antibody; EC 2.7.11.1 antibody; Never in mitosis A-related kinase 8 antibody; NimA-related protein kinase 8 antibody
Raised in
Rabbit
Species Reactivity
Mus musculus (Mouse)
Immunogen
Recombinant Mus musculus (Mouse) Nek8 protein
Immunogen Species
Mus musculus (Mouse)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia. Plays a role in organogenesis and is involved in the regulation of the Hippo signaling pathway.
Gene References into Functions
  1. NEK8 plays a critical role in replication fork stability through its regulation of the DNA repair and replication fork protection protein RAD51. PMID: 27892797
  2. NEK8-depleted inner medullary collecting duct cells exhibited a defective response to fluid shear. PMID: 23274954
  3. Nek8 acts downstream of Inv function PMID: 22687244
  4. cross-species analysis between zebrafish and mouse demonstrates taht NEK8 has a role in development of pronephric cysts PMID: 12421721
  5. Nek8 and Pkd1 genes function in a common pathway to regulate cystogenesis. Moreover, compound Pkd1 and Nek8 heterozygous adult mice develop a more aggressive cystic disease than animals with a mutation in either gene alone. PMID: 17928412
  6. Defects in ciliary localization of Nek8 is associated with polycystic kidney diseases PMID: 18189147
  7. interacts with the signal transduction pathways of the polycystins and may lead to cystogenesis by altering the structure and function of cilia in the distal nephron PMID: 18235101

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Involvement in disease
Defects in Nek8 are the cause of autosomal recessive juvenile polycystic kidney disease (ARJPKD).
Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton. Cell projection, cilium. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Protein Families
Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily
Tissue Specificity
Kidney, liver, and testis.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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