Recombinant Mouse Serine/threonine-protein kinase Nek8 (Nek8)

Code CSB-YP849695MO
MSDS
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Source Yeast
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Code CSB-EP849695MO
MSDS
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Source E.coli
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Code CSB-EP849695MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP849695MO
MSDS
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Source Baculovirus
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Code CSB-MP849695MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nek8
Uniprot No.
Alternative Names
Nek8; Jck; Serine/threonine-protein kinase Nek8; EC 2.7.11.1; Never in mitosis A-related kinase 8; NimA-related protein kinase 8
Species
Mus musculus (Mouse)
Expression Region
1-698
Target Protein Sequence
MEKYERIRVV GRGAFGIVHL CLRKADQKLV ILKQIPVEQM TKEERQAAQN ECQVLKLLNH PNVIEYYENF LEDKALMIAM EYAPGGTLAE FIQKRCNSLL EEETILHFFV QILLALHHVH THLILHRDLK TQNILLDKHR MVVKIGDFGI SKILSSKSKA YTVVGTPCYI SPELCEGKPY NQKSDIWALG CVLYELASLK RAFEAANLPA LVLKIMSGTF APISDRYSPE LRQLVLSLLS LEPAQRPPLS HIMAQPLCIR ALLNIHTDVG SVRMRRAEKS LTPGPPIASG STGSRATSAR CRGVPRGPVR PAIPPPLSSV YAWGGGLSSP LRLPMLNTEV VQVAAGRTQK AGVTRSGRLI LWEAPPLGAG GGTLLPGAVE LPQPQFVSRF LEGQSGVTIK HVACGDLFTA CLTDRGIIMT FGSGSNGCLG HGNLTDISQP TIVEALLGYE MVQVACGASH VLALSTDGEL FAWGRGDGGR LGLGTRESHN CPQQVPVAPG QEAQRVVCGI DSSMILTSPG RVLACGSNRF NKLGLDHLSL DEEPVPYQQV EEALSFTPLG SAPLDQEPLL CVDLGTAHSA AITASGDCYT FGSNQHGQLG TSSRRVSRAP CRVQGLEGIK MVMVACGDAF TVAVGAEGEV YSWGKGTRGR LGRRDEDAGL PRPVQLDETH PYMVTSVSCC HGNTLLAVRS VTDEPVPP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia. Plays a role in organogenesis and is involved in the regulation of the Hippo signaling pathway.
Gene References into Functions
  1. NEK8 plays a critical role in replication fork stability through its regulation of the DNA repair and replication fork protection protein RAD51. PMID: 27892797
  2. NEK8-depleted inner medullary collecting duct cells exhibited a defective response to fluid shear. PMID: 23274954
  3. Nek8 acts downstream of Inv function PMID: 22687244
  4. cross-species analysis between zebrafish and mouse demonstrates taht NEK8 has a role in development of pronephric cysts PMID: 12421721
  5. Nek8 and Pkd1 genes function in a common pathway to regulate cystogenesis. Moreover, compound Pkd1 and Nek8 heterozygous adult mice develop a more aggressive cystic disease than animals with a mutation in either gene alone. PMID: 17928412
  6. Defects in ciliary localization of Nek8 is associated with polycystic kidney diseases PMID: 18189147
  7. interacts with the signal transduction pathways of the polycystins and may lead to cystogenesis by altering the structure and function of cilia in the distal nephron PMID: 18235101

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Involvement in disease
Defects in Nek8 are the cause of autosomal recessive juvenile polycystic kidney disease (ARJPKD).
Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton. Cell projection, cilium. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Protein Families
Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily
Tissue Specificity
Kidney, liver, and testis.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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