Nelf-E Antibody

Code CSB-PA019521XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Nelf-E Polyclonal antibody
Uniprot No.
Target Names
Nelf-E
Alternative Names
Nelf-E antibody; anon-66Da antibody; CG5994 antibody; Negative elongation factor E antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Nelf-E protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex, causes transcriptional pausing.
Gene References into Functions
  1. biochemical analysis reveals that the sequence-specific transcription factor, GAF, orchestrates efficient pausing by recruiting NELF to promoters before transcription initiation and by assisting in loading NELF onto Pol II after initiation PMID: 23746353
  2. The finding that both phenotypes of arrested embryos are obtained in embryos that lack maternally provided NELF-A as well as in embryos with reduced levels of maternal NELF-E show that these phenotypes result from the reduced activity of the NELF complex. PMID: 20634899
  3. propose that NELF and DSIF cause polymerase to pause in the promoter proximal region of hsp70 PMID: 12782658
Subcellular Location
Nucleus. Chromosome. Note=Associates with polytene chromosomes. Associates with the hsp70 promoter when it is inactive, but not when it is activated. The NELF complex possibly dissociates from chromatin following phosphorylation of RNA polymerase II.
Protein Families
RRM NELF-E family
Database Links

KEGG: dme:Dmel_CG5994

STRING: 7227.FBpp0076374

UniGene: Dm.20046

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