Recombinant Drosophila melanogaster Negative elongation factor E (Nelf-E)

Code CSB-YP019521DLU
MSDS
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Source Yeast
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Code CSB-EP019521DLU
MSDS
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Source E.coli
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Code CSB-EP019521DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP019521DLU
MSDS
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Source Baculovirus
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Code CSB-MP019521DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nelf-E
Uniprot No.
Alternative Names
Nelf-E; anon-66Da; CG5994; Negative elongation factor E
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-280
Target Protein Sequence
MVYIHFPNNL TEEEQMLQAK YQKLKKKKKA LQAHKAPKPE PESSLTLKRP TDARDAREVA RKLIKSGAIP AIQKQTKQDQ TSFKRPKGQE RAKRSTSETT VASYQPFSST QNDVAQETII SEIIKEEPRR QNLYQHFATE RDREERGMPE KVPMDTAQPE KPRAGNTIFV SGNKVTEDFL KKTFNDYGTI VNVSMEIEKS RGFVSFAKPE SADRAIAEIH GKNVNGINLQ VQLARRQPQI EPINDASSSA VWSSIAASKS QKGSHKDHRE MVQYDEDFLL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex, causes transcriptional pausing.
Gene References into Functions
  1. biochemical analysis reveals that the sequence-specific transcription factor, GAF, orchestrates efficient pausing by recruiting NELF to promoters before transcription initiation and by assisting in loading NELF onto Pol II after initiation PMID: 23746353
  2. The finding that both phenotypes of arrested embryos are obtained in embryos that lack maternally provided NELF-A as well as in embryos with reduced levels of maternal NELF-E show that these phenotypes result from the reduced activity of the NELF complex. PMID: 20634899
  3. propose that NELF and DSIF cause polymerase to pause in the promoter proximal region of hsp70 PMID: 12782658
Subcellular Location
Nucleus. Chromosome. Note=Associates with polytene chromosomes. Associates with the hsp70 promoter when it is inactive, but not when it is activated. The NELF complex possibly dissociates from chromatin following phosphorylation of RNA polymerase II.
Protein Families
RRM NELF-E family
Database Links

KEGG: dme:Dmel_CG5994

STRING: 7227.FBpp0076374

UniGene: Dm.20046

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