nhr-25 Antibody

Code CSB-PA619292XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans nhr-25 Polyclonal antibody
Uniprot No.
Target Names
nhr-25
Alternative Names
nhr-25 antibody; F11C1.6 antibody; Nuclear hormone receptor family member nhr-25 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans nhr-25 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Orphan nuclear receptor and probable transcription activator, required during development. Plays a role in male tail tip morphogenesis regulating the expression of the transcription factor dmd-3 in a negative feedback loop. Regulates vulval precursor cell (VPC) differentiation, in concert with homeobox protein lin-39. Involved in promoting embryogenesis, in concert with homeobox protein nob-1.
Gene References into Functions
  1. these data indicate that inactivation of either acs-3 or nhr-25 causes stress sensitivity and increased expression of innate immunity/stress genes, most likely by different mechanisms. PMID: 24651852
  2. We conclude that sumoylation operates at multiple levels to affect NHR-25 activity in a highly coordinated spatial and temporal manner PMID: 24348269
  3. Functional validation of a subset of the target genes showed both activating and inhibitory roles of NHR-25 and ELT-3 in regulating their targets. PMID: 24097988
  4. ACS-3-derived long-chain fatty acyl-CoAs, perhaps incorporated into complex ligands such as phosphoinositides, modulate NHR-25 function, which in turn regulates an endocrine program of lipid uptake and synthesis. PMID: 20889131
  5. Results indicate that nhr-25 plays an integral role in regulating cellular processes of epidermal cells. PMID: 15314147
  6. NHR-25 knockdown restores DTC formation and fertility in pop-1 and sys-1 mutants, suggesting that a balance between NHR-25 and beta-catenin pathway activities is required to establish both proximal and distal fates. PMID: 16890160

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Subcellular Location
Nucleus.
Protein Families
Nuclear hormone receptor family
Tissue Specificity
Expressed in the epidermis, the developing somatic gonad, and a subset of other epithelial cells.
Database Links

KEGG: cel:CELE_F11C1.6

STRING: 6239.F11C1.6a

UniGene: Cel.7338

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