Recombinant Nuclear hormone receptor family member nhr-25 (nhr-25), partial

Code CSB-YP619292CXY
MSDS
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Source Yeast
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Code CSB-EP619292CXY
MSDS
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Source E.coli
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Code CSB-EP619292CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP619292CXY
MSDS
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Source Baculovirus
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Code CSB-MP619292CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
nhr-25
Uniprot No.
Alternative Names
nhr-25; F11C1.6; Nuclear hormone receptor family member nhr-25
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Orphan nuclear receptor and probable transcription activator, required during development. Plays a role in male tail tip morphogenesis regulating the expression of the transcription factor dmd-3 in a negative feedback loop. Regulates vulval precursor cell (VPC) differentiation, in concert with homeobox protein lin-39. Involved in promoting embryogenesis, in concert with homeobox protein nob-1.
Gene References into Functions
  1. these data indicate that inactivation of either acs-3 or nhr-25 causes stress sensitivity and increased expression of innate immunity/stress genes, most likely by different mechanisms. PMID: 24651852
  2. We conclude that sumoylation operates at multiple levels to affect NHR-25 activity in a highly coordinated spatial and temporal manner PMID: 24348269
  3. Functional validation of a subset of the target genes showed both activating and inhibitory roles of NHR-25 and ELT-3 in regulating their targets. PMID: 24097988
  4. ACS-3-derived long-chain fatty acyl-CoAs, perhaps incorporated into complex ligands such as phosphoinositides, modulate NHR-25 function, which in turn regulates an endocrine program of lipid uptake and synthesis. PMID: 20889131
  5. Results indicate that nhr-25 plays an integral role in regulating cellular processes of epidermal cells. PMID: 15314147
  6. NHR-25 knockdown restores DTC formation and fertility in pop-1 and sys-1 mutants, suggesting that a balance between NHR-25 and beta-catenin pathway activities is required to establish both proximal and distal fates. PMID: 16890160

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Subcellular Location
Nucleus.
Protein Families
Nuclear hormone receptor family
Tissue Specificity
Expressed in the epidermis, the developing somatic gonad, and a subset of other epithelial cells.
Database Links

KEGG: cel:CELE_F11C1.6

STRING: 6239.F11C1.6a

UniGene: Cel.7338

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