nhr-6 Antibody

Code CSB-PA334901XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans nhr-6 Polyclonal antibody
Uniprot No.
Target Names
nhr-6
Alternative Names
nhr-6 antibody; cnr-8 antibody; nr4a5 antibody; C48D5.1Nuclear hormone receptor family member nhr-6 antibody; Nuclear receptor subfamily 4 group A member 5 antibody; Steroid hormone receptor family member cnr8 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans nhr-6 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcriptional activator that induces gene expression by binding to the NGFI-B response element (NBRE) 5'-AAAGGTCA-3'. Required for proper morphogenesis of the spermatheca and the spermatheca-uterine valve formation. Promotes cell proliferation and differentiation of the spermatheca precursor cells during spermatheca development in larval stage L4. Might play a role in promoting G1/S phase progression in the spermatheca precursor cell lineage. Also required for the differentiation of the spermatheca-uterine junction core (sujc) cells which are generating the spermatheca-uterine valve.
Gene References into Functions
  1. Genetic suppression of the proliferation phenotype does not affect the differentiation phenotypes observed in nhr-6 mutants, indicating a dualistic role for nhr-6 in regulating cell proliferation and cell differentiation during spermatheca organogenesis. PMID: 25529479
  2. Study demonstrates an in vivo requirement for NHR-6, an NR4A nuclear receptor ortholog, DNA-binding domain in a whole organism. PMID: 20506374
Subcellular Location
Nucleus.
Protein Families
Nuclear hormone receptor family, NR4 subfamily
Tissue Specificity
In hermaphrodites, expressed in the developing spermatheca and dorsal uterus. Expression includes the 8 cells of the dorsal somatic gonad primordium and the sujc cells that form the core of the spermatheca-uterine valve. Expressed in the precursor cells o
Database Links

KEGG: cel:CELE_C48D5.1

STRING: 6239.C48D5.1a

UniGene: Cel.19471

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