Recombinant Nuclear hormone receptor family member nhr-6 (nhr-6), partial

Code CSB-YP334901CXY
MSDS
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Source Yeast
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Code CSB-EP334901CXY
MSDS
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Source E.coli
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Code CSB-EP334901CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP334901CXY
MSDS
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Source Baculovirus
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Code CSB-MP334901CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
nhr-6
Uniprot No.
Alternative Names
nhr-6; cnr-8; nr4a5; C48D5.1Nuclear hormone receptor family member nhr-6; Nuclear receptor subfamily 4 group A member 5; Steroid hormone receptor family member cnr8
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional activator that induces gene expression by binding to the NGFI-B response element (NBRE) 5'-AAAGGTCA-3'. Required for proper morphogenesis of the spermatheca and the spermatheca-uterine valve formation. Promotes cell proliferation and differentiation of the spermatheca precursor cells during spermatheca development in larval stage L4. Might play a role in promoting G1/S phase progression in the spermatheca precursor cell lineage. Also required for the differentiation of the spermatheca-uterine junction core (sujc) cells which are generating the spermatheca-uterine valve.
Gene References into Functions
  1. Genetic suppression of the proliferation phenotype does not affect the differentiation phenotypes observed in nhr-6 mutants, indicating a dualistic role for nhr-6 in regulating cell proliferation and cell differentiation during spermatheca organogenesis. PMID: 25529479
  2. Study demonstrates an in vivo requirement for NHR-6, an NR4A nuclear receptor ortholog, DNA-binding domain in a whole organism. PMID: 20506374
Subcellular Location
Nucleus.
Protein Families
Nuclear hormone receptor family, NR4 subfamily
Tissue Specificity
In hermaphrodites, expressed in the developing spermatheca and dorsal uterus. Expression includes the 8 cells of the dorsal somatic gonad primordium and the sujc cells that form the core of the spermatheca-uterine valve. Expressed in the precursor cells o
Database Links

KEGG: cel:CELE_C48D5.1

STRING: 6239.C48D5.1a

UniGene: Cel.19471

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