Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) NHX1 Polyclonal antibody

Uniprot No.

Q04121

Target Names

NHX1

Alternative Names

NHX1 antibody; NHA2 antibody; VPL27 antibody; VPS44 antibody; YDR456W antibody; D9461.40 antibody; Endosomal/prevacuolar sodium/hydrogen exchanger antibody; Endosomal/prevacuolar Na(+)/H(+) exchanger antibody; Vacuolar protein sorting-associated protein 44 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) NHX1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Endosomal/prevacuolar electroneutral Na(+)/H(+) exchanger which mediates intracellular sequestration of Na(+) cations, regulates vacuolar pH and contributes to osmotolerance following sudden exposure to hyperosmotic media. Contributes also to the postdiauxic/stationary phase resistance to osmotic stress and allows for the continued growth of cells until the acquired osmotolerance response can occur. Involved in hygromycin resistance probably through its influence on the electrochemical proton gradient affecting secondarily the entrance of hygromycin. Mediates pH-dependent vesicle trafficking out of the endosome. Contributes to K(+) sequestration and homeostasis.

Gene References into Functions

Data suggest that Nhx1p plays an important role in regulation of the luminal pH of various intracellular organelles, and that this regulation is critical for the protein recycling pathway as well as the endocytic pathway. PMID: 22210050
Nhx1 is not required for cargo sorting or multivesicular body formation and shows synthetic phenotypes with select endosomal sorting complex required for transport mutants PMID: 21998311
Nhx1p contributes to MVB formation by the recruitment of Vps27p to the endosomal membrane, possibly through Nhx1p antiporter activity. PMID: 21896492
The authors conclude that Arl1p, Kha1p and Nhx1p collaborate in survival strategies at nonoptimal pH, temperatures and cation concentrations, but work independent of each other. PMID: 20659170
Gene knockout of NHX1 increases sensitivity to hygromycin and acidic pH in yeast cells. PMID: 20858221
the Na+K+/H+ exchanger Nhx1 regulates cellular pH to control vesicle trafficking PMID: 15635088
The trafficking phenotypes of all Nhx1 mutants, including hygromycin-sensitivity and missorting of carboxypeptidase Y, were found to directly correlate with pH homoeostasis defects and could be proportionately corrected by titration with weak base. PMID: 16671892
The high sequence conservation within this subfamily of yeast antiporters suggests that Nhx1p is of great importance in cell physiology PMID: 17176761

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Subcellular Location

Endosome membrane; Multi-pass membrane protein. Prevacuolar compartment membrane; Multi-pass membrane protein.

Protein Families

Monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family

Database Links

KEGG: sce:YDR456W

STRING: 4932.YDR456W

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NHX1 Antibody

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