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Recombinant Saccharomyces cerevisiae Endosomal/prevacuolar sodium/hydrogen exchanger (NHX1), partial

Recombinant Saccharomyces cerevisiae Endosomal/prevacuolar sodium/hydrogen exchanger (NHX1), partial

Code	CSB-YP312895SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP312895SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP312895SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP312895SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP312895SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

NHX1

Uniprot No.

Q04121

Alternative Names

NHX1; NHA2; VPL27; VPS44; YDR456W; D9461.40; Endosomal/prevacuolar sodium/hydrogen exchanger; Endosomal/prevacuolar Na(+)/H(+) exchanger; Vacuolar protein sorting-associated protein 44

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Endosomal/prevacuolar electroneutral Na(+)/H(+) exchanger which mediates intracellular sequestration of Na(+) cations, regulates vacuolar pH and contributes to osmotolerance following sudden exposure to hyperosmotic media. Contributes also to the postdiauxic/stationary phase resistance to osmotic stress and allows for the continued growth of cells until the acquired osmotolerance response can occur. Involved in hygromycin resistance probably through its influence on the electrochemical proton gradient affecting secondarily the entrance of hygromycin. Mediates pH-dependent vesicle trafficking out of the endosome. Contributes to K(+) sequestration and homeostasis.

Gene References into Functions

Data suggest that Nhx1p plays an important role in regulation of the luminal pH of various intracellular organelles, and that this regulation is critical for the protein recycling pathway as well as the endocytic pathway. PMID: 22210050
Nhx1 is not required for cargo sorting or multivesicular body formation and shows synthetic phenotypes with select endosomal sorting complex required for transport mutants PMID: 21998311
Nhx1p contributes to MVB formation by the recruitment of Vps27p to the endosomal membrane, possibly through Nhx1p antiporter activity. PMID: 21896492
The authors conclude that Arl1p, Kha1p and Nhx1p collaborate in survival strategies at nonoptimal pH, temperatures and cation concentrations, but work independent of each other. PMID: 20659170
Gene knockout of NHX1 increases sensitivity to hygromycin and acidic pH in yeast cells. PMID: 20858221
the Na+K+/H+ exchanger Nhx1 regulates cellular pH to control vesicle trafficking PMID: 15635088
The trafficking phenotypes of all Nhx1 mutants, including hygromycin-sensitivity and missorting of carboxypeptidase Y, were found to directly correlate with pH homoeostasis defects and could be proportionately corrected by titration with weak base. PMID: 16671892
The high sequence conservation within this subfamily of yeast antiporters suggests that Nhx1p is of great importance in cell physiology PMID: 17176761