NHX7 Antibody

Code CSB-PA867961XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NHX7 Polyclonal antibody
Uniprot No.
Target Names
NHX7
Alternative Names
NHX7 antibody; SOS1 antibody; At2g01980 antibody; F14H20.5 antibody; Sodium/hydrogen exchanger 7 antibody; Na(+)/H(+) exchanger 7 antibody; NHE-7 antibody; Protein SALT OVERLY SENSITIVE 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana NHX7 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acts in electroneutral exchange of protons for cations such as Na(+) or Li(+) across plasma membrane. Involved in Na(+) and K(+) homeostasis. Required for cytoplasmic Na(+) and Li(+) detoxification by secreting them from the cytoplasm to the extracellular space. Regulates Na(+) content of the xylem sap.
Gene References into Functions
  1. Co-overexpression of AtNHX1 and SOS1 could significantly reduce yield loss caused by the combined stresses of heat and salt, confirming the hypothesis that stacked overexpression of two genes could substantially improve tolerance against multiple stresses. PMID: 26985021
  2. Standard chromatin immunoprecipitation and reporter assays suggest that TBX2 represses Oca2 at least in part directly. Hence, the results suggest that TBX2 may act as a nexus linking cell proliferation and melanogenesis PMID: 26992907
  3. SOS1 mutant showed severe growth inhibition under salt shock stress owing to a single base deletion PMID: 23656872
  4. AtMyb73 expression was upregulated by salt stress but not by other stresses. Accumulation of salt overly sensitive (SOS) transcripts, SOS1 and SOS3, was higher in atmyb73 ko and atmyb73 eko plants than in wild type plants receiving 300mM NaCl treatment. PMID: 23809151
  5. Haem oxygenase modifies salinity tolerance in by controlling K(+) retention via regulation of the plasma membrane H(+)-ATPase and by altering SOS1 transcript levels in roots. PMID: 23307916
  6. The protein is a homodimer that contains a membrane domain similar to that found in other antiporters of the family and an elongated, large, and structured cytosolic domain. PMID: 23022605
  7. Findings demonstrate that SOS1 is required for a large subset of the AVP1OX phenotypes many of which are salt independent. PMID: 22195582
  8. SOS1 is relieved from auto-inhibition upon phosphorylation of the auto-inhibitory domain by SOS2-SOS3. PMID: 21262798
  9. SOS1 controls membrane traffic and vacuolar functions. PMID: 20054031
  10. K(+) nutrition in salt overly sensitive1 (sos1) mutants PMID: 15347782
  11. Regulates several genes related to oxidative-stress tolerance. PMID: 17023541
  12. Truncation of the hydrophilic tail relieved the toxicity and proved that this protein is an excellent low-affinity K+ and Rb+ transporter. PMID: 17103013
  13. SOS2 family of protein kinases and their activators, the SOS3 family of calcium-binding proteins, function together; SOS2 is activated by Ca-SOS3 and subsequently phosphorylates ion transporter SOS1 to protect cellular ion homeostasis under salt stress PMID: 17499048
  14. The SOS1 transporter is involved in H(+) influx into the meristem zone of Arabidopsis roots. PMID: 19758408

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family
Tissue Specificity
More expressed in roots than in shoots. Mostly localized in parenchyma cells at the xylem/symplast boundary in roots, hypocotyls, stems and leaves. Also present in root tips epidermal cells.
Database Links

KEGG: ath:AT2G01980

STRING: 3702.AT2G01980.1

UniGene: At.27246

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