Recombinant Arabidopsis thaliana Sodium/hydrogen exchanger 7 (NHX7), partial

Code CSB-YP867961DOA
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Source Yeast
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Code CSB-EP867961DOA
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Source E.coli
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Code CSB-EP867961DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP867961DOA
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Source Baculovirus
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Code CSB-MP867961DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NHX7
Uniprot No.
Alternative Names
NHX7; SOS1; At2g01980; F14H20.5; Sodium/hydrogen exchanger 7; Na(+)/H(+) exchanger 7; NHE-7; Protein SALT OVERLY SENSITIVE 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Acts in electroneutral exchange of protons for cations such as Na(+) or Li(+) across plasma membrane. Involved in Na(+) and K(+) homeostasis. Required for cytoplasmic Na(+) and Li(+) detoxification by secreting them from the cytoplasm to the extracellular space. Regulates Na(+) content of the xylem sap.
Gene References into Functions
  1. Co-overexpression of AtNHX1 and SOS1 could significantly reduce yield loss caused by the combined stresses of heat and salt, confirming the hypothesis that stacked overexpression of two genes could substantially improve tolerance against multiple stresses. PMID: 26985021
  2. Standard chromatin immunoprecipitation and reporter assays suggest that TBX2 represses Oca2 at least in part directly. Hence, the results suggest that TBX2 may act as a nexus linking cell proliferation and melanogenesis PMID: 26992907
  3. SOS1 mutant showed severe growth inhibition under salt shock stress owing to a single base deletion PMID: 23656872
  4. AtMyb73 expression was upregulated by salt stress but not by other stresses. Accumulation of salt overly sensitive (SOS) transcripts, SOS1 and SOS3, was higher in atmyb73 ko and atmyb73 eko plants than in wild type plants receiving 300mM NaCl treatment. PMID: 23809151
  5. Haem oxygenase modifies salinity tolerance in by controlling K(+) retention via regulation of the plasma membrane H(+)-ATPase and by altering SOS1 transcript levels in roots. PMID: 23307916
  6. The protein is a homodimer that contains a membrane domain similar to that found in other antiporters of the family and an elongated, large, and structured cytosolic domain. PMID: 23022605
  7. Findings demonstrate that SOS1 is required for a large subset of the AVP1OX phenotypes many of which are salt independent. PMID: 22195582
  8. SOS1 is relieved from auto-inhibition upon phosphorylation of the auto-inhibitory domain by SOS2-SOS3. PMID: 21262798
  9. SOS1 controls membrane traffic and vacuolar functions. PMID: 20054031
  10. K(+) nutrition in salt overly sensitive1 (sos1) mutants PMID: 15347782
  11. Regulates several genes related to oxidative-stress tolerance. PMID: 17023541
  12. Truncation of the hydrophilic tail relieved the toxicity and proved that this protein is an excellent low-affinity K+ and Rb+ transporter. PMID: 17103013
  13. SOS2 family of protein kinases and their activators, the SOS3 family of calcium-binding proteins, function together; SOS2 is activated by Ca-SOS3 and subsequently phosphorylates ion transporter SOS1 to protect cellular ion homeostasis under salt stress PMID: 17499048
  14. The SOS1 transporter is involved in H(+) influx into the meristem zone of Arabidopsis roots. PMID: 19758408

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family
Tissue Specificity
More expressed in roots than in shoots. Mostly localized in parenchyma cells at the xylem/symplast boundary in roots, hypocotyls, stems and leaves. Also present in root tips epidermal cells.
Database Links

KEGG: ath:AT2G01980

STRING: 3702.AT2G01980.1

UniGene: At.27246

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