Nit1 Antibody

Datasheet

Code	CSB-PA848685XA01MO
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Product Details

Full Product Name

Rabbit anti-Mus musculus (Mouse) Nit1 Polyclonal antibody

Uniprot No.

Q8VDK1

Target Names

Nit1

Alternative Names

Nit1Deaminated glutathione amidase antibody; dGSH amidase antibody; EC 3.5.1.128 antibody; Nitrilase homolog 1 antibody

Raised in

Rabbit

Species Reactivity

Mus musculus (Mouse)

Immunogen

Recombinant Mus musculus (Mouse) Nit1 protein

Immunogen Species

Mus musculus (Mouse)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Catalyzes the hydrolysis of the amide bond in N-(4-oxoglutarate)-L-cysteinylglycine (deaminated glutathione), a metabolite repair reaction to dispose of the harmful deaminated glutathione. Possesses amidase activity toward deaminated ophthalmate in vitro. Plays a role in cell growth and apoptosis: loss of expression promotes cell growth, resistance to DNA damage stress and increased incidence to NMBA-induced tumors. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells.

Gene References into Functions

Data show that cisplatin response was enhanced in human lung cancer cells when Nit1 was knocked down and mice Nit1-/-:KrasG12D/+ tumors showed increased sensitivity to cisplatin in vivo. PMID: 26967383
Nit1 and Fhit share tumor suppressor signaling pathways, while localization of the NIT1 gene at a stable chromosome site explains the paucity of gene alterations and in frequent loss of expression of the NIT1 gene in human malignancies. PMID: 16864578
genetic deficiency results in T cell hyperproliferative responses induced by TCR stimulation, spontaneous cell cycle entry and enhanced cell cycle progression PMID: 19395373
the extent of tumor susceptibility due to Nit1 and Fhit deficiency is additive PMID: 19479888

Subcellular Location

[Isoform 1]: Mitochondrion.; [Isoform 2]: Cytoplasm.

Protein Families

Carbon-nitrogen hydrolase superfamily, NIT1/NIT2 family

Tissue Specificity

Expressed in most tissues with higher expression in adult liver and kidney as well as in fetal adrenal gland and skeletal muscle.

Database Links

KEGG: mmu:27045

STRING: 10090.ENSMUSP00000106926

UniGene: Mm.12915

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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Nit1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background