Recombinant Mouse Nitrilase homolog 1 (Nit1)

Code CSB-YP848685MO
MSDS
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Source Yeast
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Code CSB-EP848685MO
MSDS
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Source E.coli
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Code CSB-EP848685MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP848685MO
MSDS
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Source Baculovirus
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Code CSB-MP848685MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nit1
Uniprot No.
Alternative Names
Nit1Deaminated glutathione amidase; dGSH amidase; EC 3.5.1.128; Nitrilase homolog 1
Species
Mus musculus (Mouse)
Expression Region
1-323
Target Protein Sequence
MLGFITRPPH QLLCTGYRLL RTPVLCTQPR PRTMSSSTSW ELPLVAVCQV TSTPNKQENF KTCAELVQEA ARLGACLAFL PEAFDFIARN PAETLLLSEP LNGDLLGQYS QLARECGIWL SLGGFHERGQ DWEQNQKIYN CHVLLNSKGS VVASYRKTHL CDVEIPGQGP MRESNYTKPG GTLEPPVKTP AGKVGLAICY DMRFPELSLK LAQAGAEILT YPSAFGSVTG PAHWEVLLRA RAIESQCYVI AAAQCGRHHE TRASYGHSMV VDPWGTVVAR CSEGPGLCLA RIDLHFLQQM RQHLPVFQHR RPDLYGSLGH PLS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the hydrolysis of the amide bond in N-(4-oxoglutarate)-L-cysteinylglycine (deaminated glutathione), a metabolite repair reaction to dispose of the harmful deaminated glutathione. Possesses amidase activity toward deaminated ophthalmate in vitro. Plays a role in cell growth and apoptosis: loss of expression promotes cell growth, resistance to DNA damage stress and increased incidence to NMBA-induced tumors. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells.
Gene References into Functions
  1. Data show that cisplatin response was enhanced in human lung cancer cells when Nit1 was knocked down and mice Nit1-/-:KrasG12D/+ tumors showed increased sensitivity to cisplatin in vivo. PMID: 26967383
  2. Nit1 and Fhit share tumor suppressor signaling pathways, while localization of the NIT1 gene at a stable chromosome site explains the paucity of gene alterations and in frequent loss of expression of the NIT1 gene in human malignancies. PMID: 16864578
  3. genetic deficiency results in T cell hyperproliferative responses induced by TCR stimulation, spontaneous cell cycle entry and enhanced cell cycle progression PMID: 19395373
  4. the extent of tumor susceptibility due to Nit1 and Fhit deficiency is additive PMID: 19479888
Subcellular Location
[Isoform 1]: Mitochondrion.; [Isoform 2]: Cytoplasm.
Protein Families
Carbon-nitrogen hydrolase superfamily, NIT1/NIT2 family
Tissue Specificity
Expressed in most tissues with higher expression in adult liver and kidney as well as in fetal adrenal gland and skeletal muscle.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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