Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NOA1 Polyclonal antibody

Uniprot No.

Q66GP9

Target Names

NOA1

Alternative Names

NOA1 antibody; NOS1 antibody; RIF1 antibody; At3g47450 antibody; T21L8.200 antibody; NO-associated protein 1 antibody; chloroplastic/mitochondrial antibody; AtNOA1 antibody; Dubious mitochondrial nitric oxide synthase 1 antibody; AtNOS1 antibody; EC 1.14.13.39 antibody; GTPase NOA1 antibody; Protein RESISTANT TO INHIBITION BY FOSMIDOMYCIN 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana NOA1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Exhibits cGTPase activity; binds and hydrolyzes specifically GTP. May participate in ribosome assembly and stability and thus regulates protein synthesis in chloroplasts. The GTPase activity requires MgCl(2)and the presence of either KCl or (NH(4))(2)SO(4). Involved in the post-transcriptional regulation of the methylerythritol phosphate (MEP) pathway. Involved in chlorophyll-a fluorescence regulation.; May mediate the production or accumulation of nitric oxide (NO) which is a messenger molecule involved in hormonal signaling and defense responses in plant. Acts as an antisenescence agent. Plays a crucial role in both extracellular calmodulin (ExtCaM)-triggered and salicylic acid (SA)-mediated H(2)O(2)-dependent stomatal closure.

Gene References into Functions

in response to carbon dioxide elevation, either the NR activity induction in low-nitrate plants or the NR activity inhibition in high-nitrate plants is regulated by NOS-generated nitric oxide. PMID: 26608644
The protein S-acyltransferases PAT13 and PAT14 are involved in leaf senescence control- possibly via NOA1 S-acylation-, providing a new sight into the regulation mechanism of S-acylation in leaf senescence. PMID: 26160582
Salicylic acid signaling through NPR1 and AtNOA1 is involved in the control of root waving. PMID: 25690466
NOA1 has a primary role in chloroplast function and its effects on the accumulation of nitric oxide are likely to be indirect. PMID: 21689173
NIA1/2/NOA1-dependent NO production mediates Arabidopsis salt tolerance. PMID: 23744476
Oleic levels regulate nitric oxide(NO) synthesis, and, thereby, NO-mediated signaling, by regulating NOA1 levels. PMID: 22492810
AtNOA1 regulates salicylic acid-induced NO production and stomatal closure. PMID: 20657186
Atnoa1 mutant has higher salicylic acid content than wild-type Arabidopsis. PMID: 21392840
A highly conserved nuclear-encoded cGTPase of likely prokaryotic origin is essential for proper chloroplast ribosome assembly and/or translation in plants. PMID: 20456051
NOA1/nitrate reductase triple mutants are severely impaired in nitric oxide production. PMID: 20007448
Atnos1 mutant plants show increased hypersensitivity to salt stress and methyl viologen (MV) treatment. PMID: 16690168
The results of studies concerning the apparent function of ATNOS1 are reported and discussed. PMID: 17030145
AtNOS1 is involved in salt tolerance in Arabidopsis. PMID: 17351048
At3g47450 was found to be a plastid-targeted homolog of the Bacillus subtilis YqeH protein, a GTPase required for proper ribosome assembly, and in mutant seedlings, decreased levels of plastome-encoded proteins were observed. PMID: 18469163
AtNOS/AtNOA1 is a functional Arabidopsis thaliana cGTPase and not a nitric-oxide synthase PMID: 18801746
YqeH and AtNOA1 probably act as G-proteins that regulate nucleic acid recognition and not as nitric-oxide synthases PMID: 18801747
NITRIC OXIDE ASSOCIATED1 (AtNOA1)-dependent nitric oxide (NO) accumulation plays a crucial role in Extracellular calmodulin-induced stomatal closure. PMID: 19321706

Hide All

Subcellular Location

Mitochondrion. Plastid, chloroplast.

Protein Families

TRAFAC class YlqF/YawG GTPase family, NOA1 subfamily

Tissue Specificity

Expressed in aleurone layer and the embryo.

Database Links

KEGG: ath:AT3G47450

STRING: 3702.AT3G47450.1

UniGene: At.43334

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