Recombinant Arabidopsis thaliana Nitric oxide synthase 1 (NOS1), partial

Code CSB-YP721062DOA
MSDS
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Source Yeast
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Code CSB-EP721062DOA
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Source E.coli
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Code CSB-EP721062DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP721062DOA
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Source Baculovirus
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Code CSB-MP721062DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NOA1
Uniprot No.
Alternative Names
NOA1; NOS1; RIF1; At3g47450; T21L8.200; NO-associated protein 1; chloroplastic/mitochondrial; AtNOA1; Dubious mitochondrial nitric oxide synthase 1; AtNOS1; EC 1.14.13.39; GTPase NOA1; Protein RESISTANT TO INHIBITION BY FOSMIDOMYCIN 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Exhibits cGTPase activity; binds and hydrolyzes specifically GTP. May participate in ribosome assembly and stability and thus regulates protein synthesis in chloroplasts. The GTPase activity requires MgCl(2)and the presence of either KCl or (NH(4))(2)SO(4). Involved in the post-transcriptional regulation of the methylerythritol phosphate (MEP) pathway. Involved in chlorophyll-a fluorescence regulation.; May mediate the production or accumulation of nitric oxide (NO) which is a messenger molecule involved in hormonal signaling and defense responses in plant. Acts as an antisenescence agent. Plays a crucial role in both extracellular calmodulin (ExtCaM)-triggered and salicylic acid (SA)-mediated H(2)O(2)-dependent stomatal closure.
Gene References into Functions
  1. in response to carbon dioxide elevation, either the NR activity induction in low-nitrate plants or the NR activity inhibition in high-nitrate plants is regulated by NOS-generated nitric oxide. PMID: 26608644
  2. The protein S-acyltransferases PAT13 and PAT14 are involved in leaf senescence control- possibly via NOA1 S-acylation-, providing a new sight into the regulation mechanism of S-acylation in leaf senescence. PMID: 26160582
  3. Salicylic acid signaling through NPR1 and AtNOA1 is involved in the control of root waving. PMID: 25690466
  4. NOA1 has a primary role in chloroplast function and its effects on the accumulation of nitric oxide are likely to be indirect. PMID: 21689173
  5. NIA1/2/NOA1-dependent NO production mediates Arabidopsis salt tolerance. PMID: 23744476
  6. Oleic levels regulate nitric oxide(NO) synthesis, and, thereby, NO-mediated signaling, by regulating NOA1 levels. PMID: 22492810
  7. AtNOA1 regulates salicylic acid-induced NO production and stomatal closure. PMID: 20657186
  8. Atnoa1 mutant has higher salicylic acid content than wild-type Arabidopsis. PMID: 21392840
  9. A highly conserved nuclear-encoded cGTPase of likely prokaryotic origin is essential for proper chloroplast ribosome assembly and/or translation in plants. PMID: 20456051
  10. NOA1/nitrate reductase triple mutants are severely impaired in nitric oxide production. PMID: 20007448
  11. Atnos1 mutant plants show increased hypersensitivity to salt stress and methyl viologen (MV) treatment. PMID: 16690168
  12. The results of studies concerning the apparent function of ATNOS1 are reported and discussed. PMID: 17030145
  13. AtNOS1 is involved in salt tolerance in Arabidopsis. PMID: 17351048
  14. At3g47450 was found to be a plastid-targeted homolog of the Bacillus subtilis YqeH protein, a GTPase required for proper ribosome assembly, and in mutant seedlings, decreased levels of plastome-encoded proteins were observed. PMID: 18469163
  15. AtNOS/AtNOA1 is a functional Arabidopsis thaliana cGTPase and not a nitric-oxide synthase PMID: 18801746
  16. YqeH and AtNOA1 probably act as G-proteins that regulate nucleic acid recognition and not as nitric-oxide synthases PMID: 18801747
  17. NITRIC OXIDE ASSOCIATED1 (AtNOA1)-dependent nitric oxide (NO) accumulation plays a crucial role in Extracellular calmodulin-induced stomatal closure. PMID: 19321706

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Subcellular Location
Mitochondrion. Plastid, chloroplast.
Protein Families
TRAFAC class YlqF/YawG GTPase family, NOA1 subfamily
Tissue Specificity
Expressed in aleurone layer and the embryo.
Database Links

KEGG: ath:AT3G47450

STRING: 3702.AT3G47450.1

UniGene: At.43334

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