Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) NPF Polyclonal antibody

Uniprot No.

Q9VET0

Target Names

NPF

Alternative Names

NPF antibody; CG10342 antibody; Neuropeptide F antibody; NPF antibody; dNPF antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) NPF protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Integral part of the sensory system that mediates food signaling, providing the neural basis for the regulation of food response; coordinates larval foraging and social behavior changes during development. Required in dopaminergic (DA) neurons that innervate the mushroom body for satiety to suppress appetitive memory performance; a key factor in the internal state of hunger in the brain. NPF neurons coordinately modulate diverse sensory and motor neurons important for feeding, flight, and locomotion. NPF/NPFR pathway exerts its suppressive effect on larval aversion to diverse stressful stimuli (chemical stress and noxious heat) through attenuation of TRP channel-induced neuronal excitation. NPF neural signaling system plays a physiological role in acute modulation of alcohol sensitivity in adults, rather than a general response to intoxication by sedative agents. Activation and inhibition of the NPF system reduces and enhances ethanol preference, respectively. Sexual experience, the NPF system activity and ethanol consumption are all linked; sexual deprivation is a major contributor to enhanced ethanol preference.

Gene References into Functions

Flies in which neuropeptide F (NPF) neurons are activated display preference for the illuminated side of the chamber. We show that optogenetic activation of NPF neuron is rewarding in olfactory conditioning experiments and that the preference for NPF neuron activation is dependent on NPF signaling. PMID: 28874527
NPF-expressing cells, specifically those expressing the circadian photoreceptor cryptochrome, are largely responsible for changes to sleep behavior caused by NPF neuron activation PMID: 28636933
In Drosophila melanogaster neuropeptide F (NPF) modulates the responses of a specific population of antennal olfactory sensory neurons (OSNs) to food-derived odors. Knock-down of NPF in NPF neurons specifically reduces the responses of the ab3A neurons to ethyl butyrate, a volatile ester found in apples and other fruits. PMID: 28482894
These data highlight the importance of neuronal clocks for peripheral rhythms, particularly in a specific detoxification pathway, and identify a novel and conserved role for NPF/Npy in circadian rhythms. PMID: 27077948
Too much or too little NPF or its shorter isoform, which are key players in homeostatic feeding regulation, leads to increased sensitivity to amino acid starvation and diminished survivorship when compared to controls. PMID: 26909968
NPF signaling is involved in the circadian locomotor rhythm in light:dark cycles. PMID: 23614491
This study identify a small subset of clock neurons in the male brain that regulate rival-induced Longer-Mating-Duration via neuropeptide signaling. PMID: 24314729
Neuropeptide F-positive clock neurons are involved in both the control of the free-running period in constant darkness and the phasing and amplitude of the evening activity in light-dark cycles. PMID: 21826659
Data show that neuropeptide F (NPF)expression is regulated in both sex-nonspecific and male-specific manners, and that NPF also plays a role in clock-controlled sexual dimorphism in adult Drosophila. PMID: 16894172
Silencing of the neuropeptide F (npf) circuit increases fly aggression, demonstrating an opposite modulation to 5-HT. PMID: 17450142
These findings reveal an uncharacterized role of PKC in NPY/NPF-mediated acute ethanol sensitivity in flies and possibly mammals. PMID: 18675322

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Subcellular Location

Secreted.

Protein Families

NPY family

Tissue Specificity

Expressed in midgut, brain lobes and ventral nerve cord of larvae. Predominantly expressed in two pairs of protocerebral neurons in the larval CNS (at protein level). Intense expression is also seen in the fan-shaped body of the central complex and two la

Database Links

KEGG: dme:Dmel_CG10342

STRING: 7227.FBpp0304074

UniGene: Dm.5729

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NPF Antibody

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