Code | CSB-PA319977XA01FQE |
Size | US$299 |
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The process of generating polyclonal antibodies against oprI involves sequential immunizations of a rabbit with recombinant Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) oprI protein (1-83aa). Once an effective antibody titer is reached, the rabbit is bled, and antibodies are purified from the serum using affinity chromatography. Subsequent assessment of the oprI antibody's functionality in ELISA and WB applications confirms its specific reactivity towards Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) oprI protein.
The Pseudomonas aeruginosa oprI proteinin plays essential roles in the bacterium's biology and interactions with its environment. As a structural component of the outer membrane, oprI contributes to the integrity and stability of this protective barrier. OprI is implicated in the defense mechanisms of Pseudomonas aeruginosa, acting as part of the outer membrane's defense against antimicrobial agents, including host immune factors and antibiotics. Additionally, oprI may function as a virulence factor, influencing the pathogenicity of Pseudomonas aeruginosa by facilitating interactions with host tissues and contributing to immune evasion.
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KEGG: pae:PA2853
STRING: 208964.PA2853