Product Details

Full Product Name

Rabbit anti-Sus scrofa (Pig) PLA2G1B Polyclonal antibody

Uniprot No.

P00592

Target Names

PLA2G1B

Alternative Names

PLA2G1B antibody; Phospholipase A2 antibody; major isoenzyme antibody; EC 3.1.1.4 antibody; Group IB phospholipase A2 antibody; Phosphatidylcholine 2-acylhydrolase 1B antibody

Raised in

Rabbit

Species Reactivity

Sus scrofa (Pig)

Immunogen

Recombinant Sus scrofa (Pig) PLA2G1B protein

Immunogen Species

Sus scrofa (Pig)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Secretory calcium-dependent phospholipase A2 that primarily targets dietary phospholipids in the intestinal tract. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. May play a role in the biosynthesis of N-acyl ethanolamines that regulate energy metabolism and inflammation in the intestinal tract. Hydrolyzes N-acyl phosphatidylethanolamines to N-acyl lysophosphatidylethanolamines, which are further cleaved by a lysophospholipase D to release N-acyl ethanolamines. May act in an autocrine and paracrine manner. Has anti-helminth activity in a process regulated by gut microbiota. Upon helminth infection of intestinal epithelia, directly affects phosphatidylethanolamine contents in the membrane of helminth larvae, likely controlling an array of phospholipid-mediated cellular processes such as membrane fusion and cell division while providing for better immune recognition, ultimately reducing larvae integrity and infectivity.

Gene References into Functions

Equilibrium unfolding of porcine pancreatic phospholipase A2 was monitored using multiple approaches. An unusually broad unfolding transition at remarkably high denaturant concentration was observed. PMID: 26117657
Porcine group IB secretory phospholipase A2 (PLA2G1B) stimulates the expression and secretion of CXC chemokine ligand 8 (CXCL8) in human neutrophils without affecting other proinflammatory cytokines. PMID: 15528384
Porcine pancreatic PLA(2) shows a long lag phase of several hours during which the enzyme binds to the bilayer surface, but only 5+/-3% of the lipids react before the onset of rapid hydrolysis. PMID: 17355873
Results describe the structural basis for bile salt inhibition of pancreatic phospholipase A2. PMID: 17434532
MMP-2/9 production is regulated by sPLA2-IB acting as a receptor ligand to activate cPLA2 PMID: 17981679
Results show that the decylsulfate epitope for the successive E i (#) complexes increasingly resembles the micellar complex formed by the binding of PLA2 to preformed micelles. PMID: 18260608
An increased sPLA(2) activity and a reduced inhibition may play an important role in the pathogenesis of ischemia-reperfusion injury of non-heart-beating donor liver grafts. PMID: 18468626
Interfacial activation of pig pancreatic IB phospholipase A(2) (PLA2) is modeled in terms of the three discrete premicellar complexes (E(i)(#), i = 1, 2, or 3) consecutively formed by the cooperative binding of a monodisperse amphiphile to the i-face PMID: 19301847

Hide All

Subcellular Location

Secreted.

Protein Families

Phospholipase A2 family

Database Links

KEGG: ssc:445525

STRING: 9823.ENSSSCP00000028002

UniGene: Ssc.16207

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PLA2G1B Antibody

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